Iron-regulated expression and membrane localization of the maga protein in magnetospirillum sp. strain AMB-1

Chikashi Nakamura, Tomoko Kikuchi, J. Grant Burgess, Tadashi Matsunaga*

*この研究の対応する著者

研究成果: Article査読

78 被引用数 (Scopus)

抄録

The magA gene from Magnetospirillum sp. strain AMB-1 is required for the synthesis of bacterial magnetic particles (BMPs). This gene has been cloned, sequenced and found to encode a protein which is homologous to the Escherichia coli potassium efflux membrane-binding protein, KefC. By using the firefly luciferase gene (luc) cloned downstream of the magA promoter, the effect of iron on regulation of magA expression was investigated, and transcription of magA was found to be enhanced by low concentrations of iron. Intracellular localization of the MagA protein was studied using magA-luc fusion proteins. The luc gene was cloned downstream of the magA hydrophilic C-terminal domain. Detection of luciferase activity in the cytoplasm, cell membrane, and magnetic particle membrane subcellular fractions confirmed that the MagA fusion protein was localized in the cell membrane. The fusion protein was also detected on the surface of the lipid bilayer covering the magnetic particles. These results suggest that MagA is a membrane-bound protein, the expression of which is enhanced at low iron concentrations.

本文言語English
ページ(範囲)23-27
ページ数5
ジャーナルJournal of Biochemistry
118
1
出版ステータスPublished - 1995 7
外部発表はい

ASJC Scopus subject areas

  • 統計学、確率および不確実性
  • 応用数学
  • 生理学(医学)
  • 放射線学、核医学およびイメージング
  • 分子生物学
  • 生化学

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