Is your ribozyme design really correct? A proposal of simple single turnover competition assay to evaluate ribozymes

Terumichi Tanaka, Osamu Inui, Natsuki Dohi, Noriko Okada, Hidechika Okada, Yo Kikuchi

研究成果: Article

2 引用 (Scopus)

抄録

Today, many nucleic acid enzymes are used in gene therapy and gene regulations. However, no simple assay methods to evaluate enzymatic activities, with which we judge the enzyme design, have been reported. Here, we propose a new simple competition assay for nucleic acid enzymes of different types to evaluate the cleaving efficiency of a target RNA molecule, of which the recognition sites are different but overlapped. Two nucleic acid enzymes were added to one tube to make a competition of these two enzymes for one substrate. The assay was used on two ribozymes, hammerhead ribozyme and hairpin ribozyme, and a DNA-enzyme. We found that this assay method is capable of application to those enzymes, as a powerful tool for the selection and designing of RNA-cleaving enzymes.

元の言語English
ページ(範囲)1636-1644
ページ数9
ジャーナルBioscience, Biotechnology and Biochemistry
65
発行部数7
DOI
出版物ステータスPublished - 2001 7
外部発表Yes

Fingerprint

Catalytic RNA
Assays
Enzymes
assays
enzymes
Nucleic acids
Nucleic Acids
nucleic acids
RNA
Gene therapy
gene therapy
Gene expression
Genetic Therapy
DNA
Molecules
Substrates
methodology

ASJC Scopus subject areas

  • Food Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Chemistry (miscellaneous)
  • Applied Microbiology and Biotechnology
  • Bioengineering

これを引用

Is your ribozyme design really correct? A proposal of simple single turnover competition assay to evaluate ribozymes. / Tanaka, Terumichi; Inui, Osamu; Dohi, Natsuki; Okada, Noriko; Okada, Hidechika; Kikuchi, Yo.

:: Bioscience, Biotechnology and Biochemistry, 巻 65, 番号 7, 07.2001, p. 1636-1644.

研究成果: Article

Tanaka, Terumichi ; Inui, Osamu ; Dohi, Natsuki ; Okada, Noriko ; Okada, Hidechika ; Kikuchi, Yo. / Is your ribozyme design really correct? A proposal of simple single turnover competition assay to evaluate ribozymes. :: Bioscience, Biotechnology and Biochemistry. 2001 ; 巻 65, 番号 7. pp. 1636-1644.
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