Isolation and characterization of vasa in the frog Rana rugosa

Kazuhiro Saotome, Kota Hayashi, Noritaka Adachi, Yoriko Nakamura, Masahisa Nakamura

    研究成果: Article

    6 引用 (Scopus)

    抄録

    We cloned a cDNA encoding Vasa, a member of the DEAD (Asp-Glu-Ala-Asp) family of proteins, from the ovary of the frog Rana rugosa. Comparative alignment of amino acid sequences with known Vasa from several species of vertebrate showed that the R. rugosa orthologue shares eight conserved regions with Vasa from other vertebrates. Vasa gene expression was restricted to the testis and ovary among ten different tissues examined. Vasa expression showed no sexual dimorphism during sex determination in R. rugosa, but became higher in the ovary thereafter. By Western blot analysis, a single Vasa band with a molecular weight of 80.9 kDa was detected. The same antibody immunohistochemically detected Vasa in a few cells in the embryonic endoderm at stage 15; the beginning of closure of neural folds, and in the cytoplasm of spermatogonia in the testis, and oocytes in the ovary of tadpoles at stage XX; marked by one or both forelegs protruded. Together, these results suggest that Vasa is a highly specific marker of germ cells and hence useful for studies of germ cell specification and function in amphibians as it already is in other species of both invertebrates and vertebrates such as Drosophila and zebrafish.

    元の言語English
    ページ(範囲)452-459
    ページ数8
    ジャーナルJournal of Experimental Zoology Part A: Ecological Genetics and Physiology
    313 A
    発行部数7
    DOI
    出版物ステータスPublished - 2010 8 1

    Fingerprint

    Ranidae
    frog
    Anura
    frogs
    Ovary
    vertebrate
    germ cell
    vertebrates
    Vertebrates
    germ cells
    testes
    Germ Cells
    Testis
    sex determination
    spermatogonia
    sexual dimorphism
    cytoplasm
    tadpoles
    Danio rerio
    amphibian

    ASJC Scopus subject areas

    • Animal Science and Zoology
    • Ecology, Evolution, Behavior and Systematics
    • Genetics
    • Physiology
    • Molecular Biology

    これを引用

    Isolation and characterization of vasa in the frog Rana rugosa. / Saotome, Kazuhiro; Hayashi, Kota; Adachi, Noritaka; Nakamura, Yoriko; Nakamura, Masahisa.

    :: Journal of Experimental Zoology Part A: Ecological Genetics and Physiology, 巻 313 A, 番号 7, 01.08.2010, p. 452-459.

    研究成果: Article

    Saotome, Kazuhiro ; Hayashi, Kota ; Adachi, Noritaka ; Nakamura, Yoriko ; Nakamura, Masahisa. / Isolation and characterization of vasa in the frog Rana rugosa. :: Journal of Experimental Zoology Part A: Ecological Genetics and Physiology. 2010 ; 巻 313 A, 番号 7. pp. 452-459.
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    abstract = "We cloned a cDNA encoding Vasa, a member of the DEAD (Asp-Glu-Ala-Asp) family of proteins, from the ovary of the frog Rana rugosa. Comparative alignment of amino acid sequences with known Vasa from several species of vertebrate showed that the R. rugosa orthologue shares eight conserved regions with Vasa from other vertebrates. Vasa gene expression was restricted to the testis and ovary among ten different tissues examined. Vasa expression showed no sexual dimorphism during sex determination in R. rugosa, but became higher in the ovary thereafter. By Western blot analysis, a single Vasa band with a molecular weight of 80.9 kDa was detected. The same antibody immunohistochemically detected Vasa in a few cells in the embryonic endoderm at stage 15; the beginning of closure of neural folds, and in the cytoplasm of spermatogonia in the testis, and oocytes in the ovary of tadpoles at stage XX; marked by one or both forelegs protruded. Together, these results suggest that Vasa is a highly specific marker of germ cells and hence useful for studies of germ cell specification and function in amphibians as it already is in other species of both invertebrates and vertebrates such as Drosophila and zebrafish.",
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