Isolation of Magnetospirillum magneticum AMB-1 mutants defective in bacterial magnetic particle synthesis by transposon mutagenesis

Aris Tri Wahyudi, Haruko Takeyama, Tadashi Matsunaga*

*この研究の対応する著者

研究成果: Article査読

20 被引用数 (Scopus)

抄録

Nonmagnetic mutants of Magnetospirillum magneticum AMB-1 were recovered following mini-Tn5 transposon mutagenesis. Transconjugants with kanamycin resistance were obtained at a frequency of 2.7 × 10-7 per recipient. Of 3327 transconjugants, 62 were defective for bacterial magnetic particle (BMP) synthesis. The frequency of independent transposition events for nonmagnetic mutants was about 1.4% in transconjugants. Further analysis of DNA sequences flanking transposon by inverted polymerase chain reaction allowed isolation of at least 10 genes or DNA sequences involved in BMP synthesis in M. magneticum AMB-1.

本文言語English
ページ(範囲)147-154
ページ数8
ジャーナルApplied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
91-93
DOI
出版ステータスPublished - 2001
外部発表はい

ASJC Scopus subject areas

  • バイオテクノロジー
  • バイオエンジニアリング
  • 生化学
  • 応用微生物学とバイオテクノロジー
  • 分子生物学

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