Isolation of Nitrospira belonging to sublineage II from a wastewater treatment plant

Norisuke Ushiki, Hirotsugu Fujitani, Yoshiteru Aoi, Satoshi Tsuneda

研究成果: Article

37 引用 (Scopus)

抄録

Nitrite oxidation is a key step in nitrogen removal in biological wastewater treatment plants. Recently, two phylogenetically different Nitrospira (sublineages I and II) have been recognized as the numerically dominant nitriteoxidizing bacteria in wastewater treatment plants. However, Nitrospira sublineage II inhabiting activated sludge was not isolated and its detailed properties were unclear. In this study, we developed a new method for the isolation of Nitrospira forming micro-colonies using a cell sorter. We obtained a novel pure strain "Nitrospira japonica" from the activated sludge. Subsequently, phylogenetic and physiological analyses revealed that Nitrospira japonica belongs to sublineage II and grew in medium containing formate. This method has the potential to isolate other uncultured microorganisms forming micro-colonies.

元の言語English
ページ(範囲)346-353
ページ数8
ジャーナルMicrobes and Environments
28
発行部数3
DOI
出版物ステータスPublished - 2013

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activated sludge
wastewater treatment
formates
nitrites
nitrite
microorganism
oxidation
phylogenetics
microorganisms
bacterium
nitrogen
phylogeny
bacteria
methodology
cells
wastewater treatment plant
method
biological wastewater treatment
removal

ASJC Scopus subject areas

  • Plant Science
  • Soil Science
  • Ecology, Evolution, Behavior and Systematics

これを引用

Isolation of Nitrospira belonging to sublineage II from a wastewater treatment plant. / Ushiki, Norisuke; Fujitani, Hirotsugu; Aoi, Yoshiteru; Tsuneda, Satoshi.

:: Microbes and Environments, 巻 28, 番号 3, 2013, p. 346-353.

研究成果: Article

Ushiki, Norisuke ; Fujitani, Hirotsugu ; Aoi, Yoshiteru ; Tsuneda, Satoshi. / Isolation of Nitrospira belonging to sublineage II from a wastewater treatment plant. :: Microbes and Environments. 2013 ; 巻 28, 番号 3. pp. 346-353.
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