Isolation of Nitrospira belonging to sublineage II from a wastewater treatment plant

Norisuke Ushiki, Hirotsugu Fujitani, Yoshiteru Aoi, Satoshi Tsuneda*

*この研究の対応する著者

研究成果: Article査読

53 被引用数 (Scopus)

抄録

Nitrite oxidation is a key step in nitrogen removal in biological wastewater treatment plants. Recently, two phylogenetically different Nitrospira (sublineages I and II) have been recognized as the numerically dominant nitriteoxidizing bacteria in wastewater treatment plants. However, Nitrospira sublineage II inhabiting activated sludge was not isolated and its detailed properties were unclear. In this study, we developed a new method for the isolation of Nitrospira forming micro-colonies using a cell sorter. We obtained a novel pure strain "Nitrospira japonica" from the activated sludge. Subsequently, phylogenetic and physiological analyses revealed that Nitrospira japonica belongs to sublineage II and grew in medium containing formate. This method has the potential to isolate other uncultured microorganisms forming micro-colonies.

本文言語English
ページ(範囲)346-353
ページ数8
ジャーナルMicrobes and Environments
28
3
DOI
出版ステータスPublished - 2013

ASJC Scopus subject areas

  • 生態、進化、行動および分類学
  • 土壌科学
  • 植物科学

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