Isolation of replicational cue elements from a library of bent DNAs of Aspergillus oryzae

Takahiro Kusakabe*, Yasushi Sugimoto, Yoshiko Hirota, Shigenobu Toné, Yutaka Kawaguchi, Katsumi Koga, Takashi Ohyama

*この研究の対応する著者

研究成果: Article査読

3 被引用数 (Scopus)

抄録

Two fragments that could function as replicational cue elements were isolated from a genomic DNA digest of Aspergillus oryzae on the basis of abnormal behavior in polyacrylamide gel electrophoresis. The vector used in this study contained a scaffold-associated region of the Drosophila melanogaster ftz gene to provide nuclear retention. Neither fragment contained a yeast ARS consensus sequence or an eukaryotic topoisomerase II binding sequence. One of the fragments showed sequence homology with the mitochondrial replication origin of Candida utilis and a portion of mitochondrial DNA of Aspergillus nidulans. This plasmid carrying the cue fragment could also replicate in HeLa and NIH3T3 cells.

本文言語English
ページ(範囲)13-19
ページ数7
ジャーナルMolecular Biology Reports
27
1
DOI
出版ステータスPublished - 2000
外部発表はい

ASJC Scopus subject areas

  • 分子生物学
  • 遺伝学

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