KaiA-stimulated KaiC phosphorylation in circadian timing loops in cyanobacteria

Hideo Iwasaki, Taeko Nishiwaki, Yohko Kitayama, Masato Nakajima, Takao Kondo

研究成果: Article

214 被引用数 (Scopus)

抄録

Cyanobacterial clock proteins KaiA and KaiC are proposed as positive and negative regulators in the autoregulatory circadian kaiBC expression, respectively. Here, we show that activation of kaiBC expression by kaiA requires KaiC, suggesting a positive feedback control in the cyanobacterial clockwork. We found that robust circadian phosphorylation of KaiC. KaiA was essential for in vivo KaiC phosphorylation and activated in vitro KaiC autophosphorylation. These effects of KaiA were attenuated by the kaiA2 long period mutation. Both the long period phenotype and the abnormal KaiC phosphorylation in this mutant were suppressed by a previously undocumented kaiC mutation. We propose that KaiAstimulated circadian KaiC phosphorylation is important for circadian timing.

本文言語English
ページ(範囲)15788-15793
ページ数6
ジャーナルProceedings of the National Academy of Sciences of the United States of America
99
24
DOI
出版ステータスPublished - 2002 11 26
外部発表はい

ASJC Scopus subject areas

  • General

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