抄録
In the cyanobacterium Synechococcus elongatus PCC 7942, the KaiA, KaiB and KaiC proteins are essential for generation of circadian rhythms. We quantitatively analyzed the intracellular dynamics of these proteins and found a circadian rhythm in the membrane/cytosolic localization of KaiB, such that KaiB interacts with a KaiA-KaiC complex during the late subjective night. KaiB-KaiC binding is accompanied by a dramatic reduction in KaiC phosphorylation and followed by dissociation of the clock protein complex(es). KaiB attenuated KaiA-enhanced phosphorylation both in vitro and in vivo. Based on these results, we propose a novel role for KaiB in a regulatory link among subcellular localization, protein-protein interactions and post-translational modification of Kai proteins in the cyanobacterial clock system.
本文言語 | English |
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ページ(範囲) | 2127-2134 |
ページ数 | 8 |
ジャーナル | EMBO Journal |
巻 | 22 |
号 | 9 |
DOI | |
出版ステータス | Published - 2003 5月 1 |
外部発表 | はい |
ASJC Scopus subject areas
- 神経科学(全般)
- 分子生物学
- 生化学、遺伝学、分子生物学(全般)
- 免疫学および微生物学(全般)