KaiB functions as an attenuator of KaiC phosphorylation in the cyanobacterial circadian clock system

Yohko Kitayama, Hideo Iwasaki, Taeko Nishiwaki, Takao Kondo

研究成果: Article査読

213 被引用数 (Scopus)

抄録

In the cyanobacterium Synechococcus elongatus PCC 7942, the KaiA, KaiB and KaiC proteins are essential for generation of circadian rhythms. We quantitatively analyzed the intracellular dynamics of these proteins and found a circadian rhythm in the membrane/cytosolic localization of KaiB, such that KaiB interacts with a KaiA-KaiC complex during the late subjective night. KaiB-KaiC binding is accompanied by a dramatic reduction in KaiC phosphorylation and followed by dissociation of the clock protein complex(es). KaiB attenuated KaiA-enhanced phosphorylation both in vitro and in vivo. Based on these results, we propose a novel role for KaiB in a regulatory link among subcellular localization, protein-protein interactions and post-translational modification of Kai proteins in the cyanobacterial clock system.

本文言語English
ページ(範囲)2127-2134
ページ数8
ジャーナルEMBO Journal
22
9
DOI
出版ステータスPublished - 2003 5 1
外部発表はい

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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