Label-free detection of tumor markers using field effect transistor (FET)-based biosensors for lung cancer diagnosis

Shanshan Cheng, Sho Hideshima, Shigeki Kuroiwa, Takuya Nakanishi, Tetsuya Osaka*

*この研究の対応する著者

研究成果: Article査読

90 被引用数 (Scopus)

抄録

Detection of tumor markers is important for cancer diagnosis. Field effect transistor (FET) has been recognized as a powerful technique for label-free, sensitive, real-time, and multifunctional biosensing. Here, we developed FET biosensors that allow the label-free detection of cytokeratin fragment 21-1 (CYFRA 21-1) and neuron-specific enolase (NSE), useful tumor markers for lung cancer type differentiation. It was found that the FET biosensor was capable of quantitatively detecting these tumor markers in both phosphate-buffered saline and human serum. Additionally, we developed a multianalyte FET biosensor for the selective multiplexed detection of CYFRA 21-1 and NSE at the same time, by integrating two antibody types on the same chip, providing a step towards the realization of sensor arrays. The multianalyte FET biosensor, as described herein, will help for lung cancer differential diagnosis with advantages of simple and rapid detection procedures, low sample consumption, and low cost.

本文言語English
ページ(範囲)329-334
ページ数6
ジャーナルSensors and Actuators, B: Chemical
212
DOI
出版ステータスPublished - 2015 6月

ASJC Scopus subject areas

  • 電子材料、光学材料、および磁性材料
  • 器械工学
  • 凝縮系物理学
  • 表面、皮膜および薄膜
  • 金属および合金
  • 電子工学および電気工学
  • 材料化学

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