Laborless, automated microfluidic tandem cell processor for visualizing intracellular molecules of mammalian cells

Tinglin Mu, Hajime Toyoda, Yuki Kimura, Masumi Yamada*, Rie Utoh, Daisuke Umeno, Minoru Seki

*この研究の対応する著者

研究成果: Article査読

2 被引用数 (Scopus)

抄録

Visualization and quantification of intracellular molecules of mammalian cells are crucial steps in clinical diagnosis, drug development, and basic biological research. However, conventional methods rely mostly on labor-intensive, centrifugation-based manual operations for exchanging the cell carrier medium and have limited reproducibility and recovery efficiency. Here we present a microfluidic cell processor that can perform four-step exchange of carrier medium, simply by introducing a cell suspension and fluid reagents into the device. The reaction time period for each reaction step, including fixation, membrane permeabilization, and staining, was tunable in the range of 2 to 15 min by adjusting the volume of the reaction tube connecting the neighboring exchanger modules. We double-stained the cell nucleus and cytoskeleton (F-actin) using the presented device with an overall reaction period of ∼30 min, achieving a high recovery ratio and high staining efficiency. Additionally, intracellular cytokine (IL-2) was visualized for T cells to demonstrate the feasibility of the device as a pretreatment system for downstream flow-cytometric analysis. The presented approach would facilitate the development of laborless, automated microfluidic systems that integrate cell processing and analysis operations and would pave a new path to high-throughput biological experiments.

本文言語English
ページ(範囲)2580-2588
ページ数9
ジャーナルAnalytical Chemistry
92
3
DOI
出版ステータスPublished - 2020 2 4
外部発表はい

ASJC Scopus subject areas

  • 分析化学

フィンガープリント

「Laborless, automated microfluidic tandem cell processor for visualizing intracellular molecules of mammalian cells」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル