A method was developed for large scale isolation of AGY-specific serine tRNA (tRNAAGYSer) from bovine heart mitochondria. By this method, 5 A250 units of tRNAAGYSer were recovered from 6.3 kg of bovine hearts.The nucleotide sequence was identical to that reported previously. tRNAAGYSer showed abnormal melting profiles, as was predicted from its unique primary sequence. Its secondary and/or tertiary structure was analyzed by nuclease digestion method. It was suggested that three extra base pairs could occur in the anticodon stem region, with one adenosine residue protruding. The T loop was quite sensitive to nuclease S1, suggesting that the T loop doesn't interact with other regions. This finding is consistent with the model proposed by Sundaralingam (1980).tRNAAGYSer was aminoacylated in vitro with only mitochondrial enzyme but not with the enzymes from E. coli and yeast. The aminoacylation rate of tRNAAGYSer with mitochondrial enzyme was much faster than that of cytosolic tRNAAGYSer, perhaps reflecting differences due to the presence and absence of the D arm of the tRNAs.
|ジャーナル||Journal of biochemistry|
|出版ステータス||Published - 1985 11|
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