Large scale isolation and some properties of AGY-specific serine tRNA from bovine heart mitochondria

Takuya Ueda, Takahisa Ohta, Kimitsuna Watanabe*

*この研究の対応する著者

研究成果: Article査読

41 被引用数 (Scopus)

抄録

A method was developed for large scale isolation of AGY-specific serine tRNA (tRNAAGYSer) from bovine heart mitochondria. By this method, 5 A250 units of tRNAAGYSer were recovered from 6.3 kg of bovine hearts.The nucleotide sequence was identical to that reported previously. tRNAAGYSer showed abnormal melting profiles, as was predicted from its unique primary sequence. Its secondary and/or tertiary structure was analyzed by nuclease digestion method. It was suggested that three extra base pairs could occur in the anticodon stem region, with one adenosine residue protruding. The T loop was quite sensitive to nuclease S1, suggesting that the T loop doesn't interact with other regions. This finding is consistent with the model proposed by Sundaralingam (1980).tRNAAGYSer was aminoacylated in vitro with only mitochondrial enzyme but not with the enzymes from E. coli and yeast. The aminoacylation rate of tRNAAGYSer with mitochondrial enzyme was much faster than that of cytosolic tRNAAGYSer, perhaps reflecting differences due to the presence and absence of the D arm of the tRNAs.

本文言語English
ページ(範囲)1275-1284
ページ数10
ジャーナルJournal of biochemistry
98
5
DOI
出版ステータスPublished - 1985 11
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 分子生物学

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