Large scale microfluidic systems for sequential trapping, labeling and content extraction of single cells

Masao Noguchi, Takahiro Arakawa, Keiko Sumitomo, Yoshinori Yamaguchi, Shuichi Shoji

研究成果: Conference contribution

抄録

Parallel and sequential manipulation of trapping, labeling and content extraction of single cell was realized using a 2-D simple flow channels. The proposed system consists of main channels, cell trapping pockets, drain channels and single cell content collection channels. We fabricated prototypes having 16 cell trapping pockets and 512 cell trapping pockets. Sequential all single cell handling for COS-1 cell was confirmed in 16 pockets type [1]. The sequential trapping and labeling were achieved in 512 pockets type. Capture rate for COS-1 cell was 83.2% and that for BAT cell was 70.3%. These devices are useful for high throughput single cell analysis.

本文言語English
ホスト出版物のタイトルTRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems
ページ628-631
ページ数4
DOI
出版ステータスPublished - 2009 12 11
イベントTRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems - Denver, CO, United States
継続期間: 2009 6 212009 6 25

出版物シリーズ

名前TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems

Conference

ConferenceTRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems
CountryUnited States
CityDenver, CO
Period09/6/2109/6/25

ASJC Scopus subject areas

  • Hardware and Architecture
  • Electrical and Electronic Engineering

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