LGP2 Expression is Enhanced by Interferon Regulatory Factor 3 in Olive Flounder, Paralichthys olivaceus

Jun ichi Hikima, Mi Kyong Yi, Maki Ohtani, Chan Yong Jung, Young Kyu Kim, Ji Young Mun, Young Rim Kim, Haruko Takeyama, Takashi Aoki, Tae Sung Jung

    研究成果: Article

    11 引用 (Scopus)

    抄録

    In innate immunity, LGP2 (laboratory of genetics and physiology 2) plays a very important role in the production of type I interferon (IFN) through recognition of cytosolic viral RNA. Although viral infection or stimulation with double-strand RNA dramatically induces expression of the LGP2 gene, the underlying transcriptional mechanism has never been studied. Here, we cloned and characterized the 5′-upstream region (-1,337 bp) of the LGP2 gene in olive flounder (Paralichthys olivaceus). Numerous canonical motifs for IFN-regulatory factors (IRFs) were found in this region, and reporter assays identified a poly I:C-responsive promoter region (-506 to -398) that regulated LGP2 transcription. Transcriptional activity of the LGP2 promoter was strongly enhanced by IRF3, which bound to IRF3 motif #3 (-480). The LGP2 promoter was also responsive to viral infection in vitro. These results suggest that LGP2 transcriptional control is crucially involved to regulated by IRF3 function after viral infection or stimulation with poly I:C.

    元の言語English
    記事番号e51522
    ジャーナルPLoS One
    7
    発行部数12
    DOI
    出版物ステータスPublished - 2012 12 10

    Fingerprint

    Interferon Regulatory Factor-3
    Flounder
    Paralichthys olivaceus
    Physiology
    Olea
    physiology
    Virus Diseases
    polyinosinic-polycytidylic acid
    Poly I-C
    promoter regions
    Genes
    Interferon Regulatory Factors
    infection
    RNA
    Interferon Type I
    Viral RNA
    interferons
    Transcription
    Genetics
    interferon regulatory factor-3

    ASJC Scopus subject areas

    • Agricultural and Biological Sciences(all)
    • Biochemistry, Genetics and Molecular Biology(all)
    • Medicine(all)

    これを引用

    Hikima, J. I., Yi, M. K., Ohtani, M., Jung, C. Y., Kim, Y. K., Mun, J. Y., ... Jung, T. S. (2012). LGP2 Expression is Enhanced by Interferon Regulatory Factor 3 in Olive Flounder, Paralichthys olivaceus. PLoS One, 7(12), [e51522]. https://doi.org/10.1371/journal.pone.0051522

    LGP2 Expression is Enhanced by Interferon Regulatory Factor 3 in Olive Flounder, Paralichthys olivaceus. / Hikima, Jun ichi; Yi, Mi Kyong; Ohtani, Maki; Jung, Chan Yong; Kim, Young Kyu; Mun, Ji Young; Kim, Young Rim; Takeyama, Haruko; Aoki, Takashi; Jung, Tae Sung.

    :: PLoS One, 巻 7, 番号 12, e51522, 10.12.2012.

    研究成果: Article

    Hikima, JI, Yi, MK, Ohtani, M, Jung, CY, Kim, YK, Mun, JY, Kim, YR, Takeyama, H, Aoki, T & Jung, TS 2012, 'LGP2 Expression is Enhanced by Interferon Regulatory Factor 3 in Olive Flounder, Paralichthys olivaceus', PLoS One, 巻. 7, 番号 12, e51522. https://doi.org/10.1371/journal.pone.0051522
    Hikima, Jun ichi ; Yi, Mi Kyong ; Ohtani, Maki ; Jung, Chan Yong ; Kim, Young Kyu ; Mun, Ji Young ; Kim, Young Rim ; Takeyama, Haruko ; Aoki, Takashi ; Jung, Tae Sung. / LGP2 Expression is Enhanced by Interferon Regulatory Factor 3 in Olive Flounder, Paralichthys olivaceus. :: PLoS One. 2012 ; 巻 7, 番号 12.
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    abstract = "In innate immunity, LGP2 (laboratory of genetics and physiology 2) plays a very important role in the production of type I interferon (IFN) through recognition of cytosolic viral RNA. Although viral infection or stimulation with double-strand RNA dramatically induces expression of the LGP2 gene, the underlying transcriptional mechanism has never been studied. Here, we cloned and characterized the 5′-upstream region (-1,337 bp) of the LGP2 gene in olive flounder (Paralichthys olivaceus). Numerous canonical motifs for IFN-regulatory factors (IRFs) were found in this region, and reporter assays identified a poly I:C-responsive promoter region (-506 to -398) that regulated LGP2 transcription. Transcriptional activity of the LGP2 promoter was strongly enhanced by IRF3, which bound to IRF3 motif #3 (-480). The LGP2 promoter was also responsive to viral infection in vitro. These results suggest that LGP2 transcriptional control is crucially involved to regulated by IRF3 function after viral infection or stimulation with poly I:C.",
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