Linearization and integration of DNA into cells preferentially occurs at intrinsically curved regions from human LINE-1 repetitive element

Takahiro Kusakabe*, Yasushi Sugimoto, Takuji Maeda, Yumiko Nakajima, Masaru Miyano, Jun Ichi Nishikawa, Shigenobu Tone, Yutaka Kawaguchi, Katsumi Koga, Takashi Ohyama

*この研究の対応する著者

研究成果: Article査読

5 被引用数 (Scopus)

抄録

A bent DNA library was constructed from human genomic DNA, from which a new clone belonging to the human LINE-1 sequence family was isolated and characterized. This clone, with a length of 378 base pairs and termed HBC-1 (human bent clone-1), contained an intrinsically occurring curved DNA structure. By permutation analysis, the center of curvature of this fragment was mapped onto the nucleotide position 886 from the 5′ terminus of the complete LINE-1 sequence. Reporter plasmids, which contain HBC-1, were effectively integrated into human chromosome, indicating that the bent DNA structure provides a preferential donor site for the integration of human LINE-1 sequences. The present finding may provide an explanation as to why some inactivated LINE-1 sequences on human chromosomes carry the deletion at their 5′ termini.

本文言語English
ページ(範囲)271-281
ページ数11
ジャーナルGene
274
1-2
DOI
出版ステータスPublished - 2001 8 22
外部発表はい

ASJC Scopus subject areas

  • 遺伝学

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