Linkers of Cell Polarity and Cell Cycle Regulation in the Fission Yeast Protein Interaction Network

Federico Vaggi, James Dodgson, Archana Bajpai, Anatole Chessel, Ferenc Jordán, Masamitsu Sato, Rafael Edgardo Carazo-Salas, Attila Csikász-Nagy*

*この研究の対応する著者

研究成果査読

10 被引用数 (Scopus)

抄録

The study of gene and protein interaction networks has improved our understanding of the multiple, systemic levels of regulation found in eukaryotic and prokaryotic organisms. Here we carry out a large-scale analysis of the protein-protein interaction (PPI) network of fission yeast (Schizosaccharomyces pombe) and establish a method to identify 'linker' proteins that bridge diverse cellular processes - integrating Gene Ontology and PPI data with network theory measures. We test the method on a highly characterized subset of the genome consisting of proteins controlling the cell cycle, cell polarity and cytokinesis and identify proteins likely to play a key role in controlling the temporal changes in the localization of the polarity machinery. Experimental inspection of one such factor, the polarity-regulating RNB protein Sts5, confirms the prediction that it has a cell cycle dependent regulation. Detailed bibliographic inspection of other predicted 'linkers' also confirms the predictive power of the method. As the method is robust to network perturbations and can successfully predict linker proteins, it provides a powerful tool to study the interplay between different cellular processes.

本文言語English
論文番号e1002732
ジャーナルPLoS Computational Biology
8
10
DOI
出版ステータスPublished - 2012 10
外部発表はい

ASJC Scopus subject areas

  • 生態、進化、行動および分類学
  • モデリングとシミュレーション
  • 生態学
  • 分子生物学
  • 遺伝学
  • 細胞および分子神経科学
  • 計算理論と計算数学

フィンガープリント

「Linkers of Cell Polarity and Cell Cycle Regulation in the Fission Yeast Protein Interaction Network」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル