TY - JOUR
T1 - Liquid Chromatography Chip with Low-Dispersion and Low-Pressure-Drop Turn Structure Utilizing a Distribution-Controlled Pillar Array
AU - Isokawa, Muneki
AU - Takatsuki, Katsuya
AU - Song, Yanting
AU - Shih, Kailing
AU - Nakanishi, Kanki
AU - Xie, Zhimin
AU - Yoon, Dong Hyun
AU - Sekiguchi, Tetsushi
AU - Funatsu, Takashi
AU - Shoji, Shuichi
AU - Tsunoda, Makoto
N1 - Funding Information:
This research was partially supported by the Japan Society for the Promotion of Science (JSPS), Grant-in-Aid for Scientific Research (C), No. 2646033; the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan through a Grant-in-Aid for Scientific Basic Research (S), No. 23226010; the Center of Innovation Program from the Japan Science and Technology Agency (JST); a Grant-in-Aid for scientific research of JSPS Fellows (to M.I., No. 15J12103); and by the Graduate Program for Leaders in Life Innovation, The University of Tokyo Life Innovation Leading Graduate School from MEXT, Japan (to M.I.).
Publisher Copyright:
© 2016 American Chemical Society.
PY - 2016/6/21
Y1 - 2016/6/21
N2 - To realize efficient, fast separations on pillar array columns with turns, a novel turn with low-dispersion and low-pressure-drop properties was developed. This "pillar-distribution-controlled" (PDC) turn was designed as a constant-radius turn filled with octagonal pillars that were arranged to control the linear velocity of the mobile phase in the radial direction. After the pillar positions were adjusted by computational fluid dynamics analysis, 27 mm long pillar array columns with two turns were fabricated on a 20 × 20 mm2 silicon glass plate. The PDC turns suppressed the sample dispersion to a similar extent as the previously developed tapered turn, and the pressure drop of the newly designed turn was reduced to ∼1/6 that of the tapered turn. Moreover, the C18-modified pillar array column with the PDC turns showed good bioanalytical applicability; five fluorescently labeled amino acids were separated in only 24 s at a linear velocity of 7.5 mm/s. The developed turn structure offers the advantages of longer pillar array columns with more turns for the fast analysis of complex samples.
AB - To realize efficient, fast separations on pillar array columns with turns, a novel turn with low-dispersion and low-pressure-drop properties was developed. This "pillar-distribution-controlled" (PDC) turn was designed as a constant-radius turn filled with octagonal pillars that were arranged to control the linear velocity of the mobile phase in the radial direction. After the pillar positions were adjusted by computational fluid dynamics analysis, 27 mm long pillar array columns with two turns were fabricated on a 20 × 20 mm2 silicon glass plate. The PDC turns suppressed the sample dispersion to a similar extent as the previously developed tapered turn, and the pressure drop of the newly designed turn was reduced to ∼1/6 that of the tapered turn. Moreover, the C18-modified pillar array column with the PDC turns showed good bioanalytical applicability; five fluorescently labeled amino acids were separated in only 24 s at a linear velocity of 7.5 mm/s. The developed turn structure offers the advantages of longer pillar array columns with more turns for the fast analysis of complex samples.
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U2 - 10.1021/acs.analchem.6b01201
DO - 10.1021/acs.analchem.6b01201
M3 - Article
AN - SCOPUS:84975794199
VL - 88
SP - 6485
EP - 6491
JO - Analytical Chemistry
JF - Analytical Chemistry
SN - 0003-2700
IS - 12
ER -