TY - GEN
T1 - Live cells-based cytotoxic sensor chip fabricated in a microfluidic system
AU - Taniguchi, Akiyoshi
PY - 2007/12/1
Y1 - 2007/12/1
N2 - We report here a live cells-based sensorchip fabricated in microfluidic channels in which several laminar flows were achieved. In addition, we established a cytotoxic sensor cell line, which was transfected with a green fluorescence protein (GFP) plasmid derived from the heat shock protein 70B' (HSP70B') promoter, which is induced by cytotoxic reagents [1-4] (Fig. 1,2). The fluorescence in the sensor cells increased in a CdCl2 dose-dependent manner in the microfluidic channels (Fig.3) [5]. In this system, cytotoxic reagents can be quantitatively detected in a quick, sensitive and high- throughput manner. The combination of sensor cells and microfluidic systems will provide an important basis for the development of micro-total analysis systems (μ-TAS) technology, and can be applied to toxicology, environmental assessment and drug screening.
AB - We report here a live cells-based sensorchip fabricated in microfluidic channels in which several laminar flows were achieved. In addition, we established a cytotoxic sensor cell line, which was transfected with a green fluorescence protein (GFP) plasmid derived from the heat shock protein 70B' (HSP70B') promoter, which is induced by cytotoxic reagents [1-4] (Fig. 1,2). The fluorescence in the sensor cells increased in a CdCl2 dose-dependent manner in the microfluidic channels (Fig.3) [5]. In this system, cytotoxic reagents can be quantitatively detected in a quick, sensitive and high- throughput manner. The combination of sensor cells and microfluidic systems will provide an important basis for the development of micro-total analysis systems (μ-TAS) technology, and can be applied to toxicology, environmental assessment and drug screening.
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U2 - 10.1109/IMNC.2007.4456327
DO - 10.1109/IMNC.2007.4456327
M3 - Conference contribution
AN - SCOPUS:47349085939
SN - 4990247248
SN - 9784990247249
T3 - Digest of Papers - Microprocesses and Nanotechnology 2007; 20th International Microprocesses and Nanotechnology Conference, MNC
SP - 508
EP - 509
BT - Digest of Papers - Microprocesses and Nanotechnology 2007; 20th International Microprocesses and Nanotechnology Conference, MNC
T2 - s20th International Microprocesses and Nanotechnology Conference, MNC 2007
Y2 - 5 November 2007 through 8 November 2007
ER -