Localization of orexin-A-like immunoreactivity in prolactin cells in the bullfrog (Rana catesbeiana) pituitary

Toshiharu Yamamoto*, Hirohumi Suzuki, Haruko Uemura, Kazutoshi Yamamoto, Sakaé Kikuyama

*この研究の対応する著者

    研究成果: Article査読

    24 被引用数 (Scopus)

    抄録

    Immunohistochemical techniques were employed to investigate the distribution of orexin-A-like immunoreactivity in the bullfrog (Rana catesbeiana) pituitary. Orexin-A-immunoreactive cells were scattered throughout the pars distalis. We found that these cells corresponded to the cells immunostained with antiserum against bullfrog prolactin (fPRL). Immunoelectron microscopic analysis indicated that an orexin-A-like substance coexisted with fPRL within secretory granules. Western blot analysis of bullfrog pituitary extract revealed that anti-human orexin-A antiserum labeled two separate bands which were not labeled with anti-fPRL antiserum. The present study has, for the first time, provided evidence of the intragranular colocalization of orexin-A-like and PRL immunoreactivities in the bullfrog pituitary.

    本文言語English
    ページ(範囲)186-192
    ページ数7
    ジャーナルGeneral and Comparative Endocrinology
    135
    2
    DOI
    出版ステータスPublished - 2004 1月 15

    ASJC Scopus subject areas

    • 内分泌学

    フィンガープリント

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