抄録
In this paper, we demonstrate the micro heater device integrated with a micro chamber for investigation on cell-cell interactions under local thermal stimulation. The temperature distribution in the micro chamber was confirmed by on-chip temperature sensors and finite element method simulation. We successfully stimulated NIH-3T3 cells by Joule heating through the micro heater and observed localized expression of heat shock proteins (HSPs) after cells were cultured in the micro chamber confluently. The temperature distribution and the localized expression area consisted. Thus, the thermal stimulation area was confined in the micro chamber. Besides, localized expression of HSPs was observed in a cell monolayer. This device will be widely applied for the study on cell-cell interactions under local stimulation.
本文言語 | English |
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ホスト出版物のタイトル | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 |
出版社 | Chemical and Biological Microsystems Society |
ページ | 645-647 |
ページ数 | 3 |
ISBN(印刷版) | 9780979806476 |
出版ステータス | Published - 2014 |
外部発表 | はい |
イベント | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States 継続期間: 2014 10 26 → 2014 10 30 |
Other
Other | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 |
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Country | United States |
City | San Antonio |
Period | 14/10/26 → 14/10/30 |
ASJC Scopus subject areas
- Control and Systems Engineering