Localized expression of heat shock protein in cell population by micro heater device

R. Ueno, F. Yesilköy, J. Brugger, Akiyoshi Taniguchi, Y. Sakai, B. J. Kim

研究成果: Conference contribution

抄録

In this paper, we demonstrate the micro heater device integrated with a micro chamber for investigation on cell-cell interactions under local thermal stimulation. The temperature distribution in the micro chamber was confirmed by on-chip temperature sensors and finite element method simulation. We successfully stimulated NIH-3T3 cells by Joule heating through the micro heater and observed localized expression of heat shock proteins (HSPs) after cells were cultured in the micro chamber confluently. The temperature distribution and the localized expression area consisted. Thus, the thermal stimulation area was confined in the micro chamber. Besides, localized expression of HSPs was observed in a cell monolayer. This device will be widely applied for the study on cell-cell interactions under local stimulation.

本文言語English
ホスト出版物のタイトル18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
出版社Chemical and Biological Microsystems Society
ページ645-647
ページ数3
ISBN(印刷版)9780979806476
出版ステータスPublished - 2014
外部発表はい
イベント18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States
継続期間: 2014 10 262014 10 30

Other

Other18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
CountryUnited States
CitySan Antonio
Period14/10/2614/10/30

ASJC Scopus subject areas

  • Control and Systems Engineering

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