Logic gate using artificial cell-membrane: NAND operation by transmembrane DNA via a biological nanopore

Hiroki Yasuga, Ryuji Kawano, Masahiro Takinoue, Yutaro Tsuji, Toshihisa Osaki, Koki Kamiya, Norihisa Miki, Shoji Takeuchi

研究成果: Conference contribution

3 被引用数 (Scopus)

抄録

This paper describes microfluidic logic gates which use DNA and biological nanopores. Single-stranded DNA (ssDNA) can pass through αHL, a biological nanopore, incorporated in bilayer lipid membranes (BLMs), whereas double-stranded DNA (dsDNA) cannot. In this study, these passing and non-passing phenomena were applied as the binary system and logic gates. Two types of ssDNA were used as inputs, while the output was obtained by electrical signals across the nanopores, which recognizes whether ssDNA passed through the nanopores or not. NAND gate was successfully demonstrated by exploiting the mechanism. The proposed approach herein is significantly different from the conventional computation using DNA in the respect that electrical signals are directly obtained as the output, which drastically facilities the microfluidic system to connect to electrical systems for fast and accurate computing. In addition, it is not required to use fluorescence, enzyme or PCR in order to obtain outputs. We believe that this method leads to a rapid computing system using biomolecules.

本文言語English
ホスト出版物のタイトルIEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013
ページ1005-1006
ページ数2
DOI
出版ステータスPublished - 2013 4 2
外部発表はい
イベントIEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013 - Taipei, Taiwan, Province of China
継続期間: 2013 1 202013 1 24

出版物シリーズ

名前Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN(印刷版)1084-6999

Conference

ConferenceIEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013
CountryTaiwan, Province of China
CityTaipei
Period13/1/2013/1/24

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Condensed Matter Physics
  • Electronic, Optical and Magnetic Materials

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