Magnesium chloride and polyamine can differentiate mouse embryonic stem cells into trophectoderm or endoderm

Jun ichi Tanase, Takehiro Yokoo, Yuuki Matsumura, Makoto Kinoshita, Yo Kikuchi, Hirofumi Suemori, Takashi Ohyama*

*この研究の対応する著者

研究成果: Article査読

2 被引用数 (Scopus)

抄録

Magnesium chloride and polyamines stabilize DNA and chromatin. Furthermore, they can induce nucleosome aggregation and chromatin condensation in vitro. To determine the effects of elevating the cation concentrations in the nucleus of a living cell, we microinjected various concentrations of mono-, di- and polyvalent cation solutions into the nuclei of mouse embryonic stem (ES) cells and traced their fates. Here, we show that an elevation of either MgCl2, spermidine or spermine concentration in the nucleus exerts a significant effect on mouse ES cells, and can differentiate a certain population of the cells into trophectoderm, a lineage that mouse ES cells do not normally generate, or endoderm. It is hypothesized that the cell differentiation was most probably caused by the condensation of chromatin including the Oct3/4 locus, which was induced by the elevated concentrations of these cations.

本文言語English
ページ(範囲)764-770
ページ数7
ジャーナルBiochemical and Biophysical Research Communications
482
4
DOI
出版ステータスPublished - 2017 1 22

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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