Maintenance of broad host range vector pKT230 in marine unicellular cyanobacteria

Koji Sode*, Masahiro Tatara, Shigeo Ogawa, Tadashi Matsunaga

*この研究の対応する著者

研究成果: Article査読

18 被引用数 (Scopus)

抄録

The host range of pKT230 in marine unicellular cyanobacteria was investigated. The broad host range vector, pKT230, was successfully transferred by transconjugation to Synechococcus sp. PCC7335, NKBG15031a and Synechocystis 7a. pKT230 was found to be maintained as an autonomous replicon in strains NKBG15031a and 7a. pKT230 stability in Synechococcus sp. NKBG15041c, in which pKT230 was found to be maintained as an autonomous replicon in our previous report [1], was also studied. In the absence of selection pressure, after 120 h of cultivation, about 50% of cells lost the plasmid. In the presence of selection pressure (kanamycin 25 μg ml-), after 120 h of cultivation, more than 90% of the cells harboured the plasmid.

本文言語English
ページ(範囲)73-78
ページ数6
ジャーナルFEMS Microbiology Letters
99
1
出版ステータスPublished - 1992 11月 15
外部発表はい

ASJC Scopus subject areas

  • 微生物学
  • 分子生物学
  • 遺伝学

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