Mal3, the fission yeast EB1 homologue, cooperates with Bub1 spindle checkpoint to prevent monopolar attachment

Kazuhide Asakawa, Mika Toya, Masamitsu Sato, Muneyoshi Kanai, Kazunori Kume, Tetsuya Goshima, Miguel Angel Garcia, Dai Hirata, Takashi Toda*

*この研究の対応する著者

研究成果: Article査読

21 被引用数 (Scopus)

抄録

Bipolar microtubule attachment is central to genome stability. Here, we investigate the mitotic role of the fission yeast EB1 homologue Mal3. Mal3 shows dynamic inward movement along the spindle, initial emergence at the spindle pole body (SPB) and translocation towards the equatorial plane, followed by sudden disappearance. Deletion of Mal3 results in early mitotic delay, which is dependent on the Bub1, but not the Mad2, spindle checkpoint. Consistently, Bub1, but not Mad2, shows prolonged kinetochore localization. Double mutants between mal3 and a subset of checkpoint mutants, including bub1, bub3, mad3 and mph1, but not mad1 or mad2, show massive chromosome mis-segregation defects. In mal3bub1 mutants, both sister centromeres tend to remain in close proximity to one of the separating SPBs. Further analysis indicates that mis-segregated centromeres are exclusively associated with the mother SPB. Mal3, therefore, has a role in preventing monopolar attachment in cooperation with the Bub1/Bub3/Mad3/Mph1-dependent checkpoint.

本文言語English
ページ(範囲)1194-1200
ページ数7
ジャーナルEMBO Reports
6
12
DOI
出版ステータスPublished - 2005 12月
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 分子生物学
  • 遺伝学

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