Malachite green-conjugated microtubules as mobile bioprobes selective for malachite green aptamers with capturing/releasing ability

Miki Hirabayashi, Shu Taira, Suzuko Kobayashi, Kaoru Konishi, Kaoru Katoh, Yuichi Hiratsuka, Masato Kodaka, Taro Q.P. Uyeda, Noboru Yumoto, Tai Kubo

研究成果: Article査読

38 被引用数 (Scopus)

抄録

We have developed a novel mobile bioprobe using a conjugate of a kinesin-driven microtubule (MT) and malachite green (MG) as a platform for capturing MG RNA aptamers. The fluorescence of MG increases when it is bound to an MG aptamer, allowing MT-MG conjugates to work as sensors of RNA transcripts containing the MG aptamer sequence. Kinesin motor proteins provide an effective driving force to create mobile bioprobes without any manipulation. Although the fluorescence of a small number of MG-binding aptamers is low, the self-organization of tubulins into MTs enables the microscopic observation of the bound aptamers by collecting them on MTs. We demonstrate that MT-MG conjugates can select target aptamers from a transcription mixture and transport them without losing their inherent motility. Because the MG aptamer binds MG in a reversible manner, MT-MG conjugates can conditionally load and unload the target aptamers. This is one advantage of this system over the molecular probes developed previously in which reversible unloading is impossible due to high-affinity binding, such as between avidin and biotin. Furthermore, an MT-MG conjugate can be used as a platform for other MG aptameric sensors with recognition regions for various target analytes optimized by further selection procedures. This is the first step to applying living systems to in vitro devices. This technique could provide a new paradigm of mobile bioprobes establishing high-throughput in vitro selection systems using microfluidic devices operating in parallel.

本文言語English
ページ(範囲)473-480
ページ数8
ジャーナルBiotechnology and bioengineering
94
3
DOI
出版ステータスPublished - 2006 6 20
外部発表はい

ASJC Scopus subject areas

  • バイオテクノロジー
  • バイオエンジニアリング
  • 応用微生物学とバイオテクノロジー

フィンガープリント

「Malachite green-conjugated microtubules as mobile bioprobes selective for malachite green aptamers with capturing/releasing ability」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル