Mass Culture of Magnetic Bacteria and Their Application to Flow Type Immunoassays

Tadashi Matsunaga, F. Tadokoro, N. Nakamura

研究成果: Article査読

61 被引用数 (Scopus)

抄録

Isolated helical shaped magnetotactic bacteria were cultured in a medium containing succinate, nitrate, and ferric malate as carbon, nitrogen, and iron sources, respectively. The magnetotactic bacteria could grow aerobically. The cells which grew aerobically had oxidase activity. An initial inoculum of 105 cells/ml was used Stationary phase was reached (1.4×109 cells/ml) after 4–5 days growth. When the cells were disrupted using ultrasonication, 2.6 mg bacterial magnetites were obtained from 1 I culture of magnetotactic bacteria. The detection of mouse IgG was carried out using FITC conjugated anti-mouse IgG immobilized on bacterial magnetites and a flow injection system with a fluorescence spectrophotometer. Relative fluorescence intensity correlated linearly with the concentration of mouse IgG in the range from 0.5-100 ng/ml and the measurements were established within 2 min using this system.

本文言語English
ページ(範囲)1557-1559
ページ数3
ジャーナルIEEE Transactions on Magnetics
26
5
DOI
出版ステータスPublished - 1990
外部発表はい

ASJC Scopus subject areas

  • 電子工学および電気工学
  • 電子材料、光学材料、および磁性材料
  • 物理学および天文学(その他)

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