Mass spectrometric screening of ligands with lower off-rate from a clicked-based pooled library

Satoshi Arai, Shota Hirosawa, Yusuke Oguchi, Madoka Suzuki, Atsushi Murata, Shin'Ichi Ishiwata, Shinji Takeoka

研究成果: Review article査読

2 被引用数 (Scopus)

抄録

This paper describes a convenient screening method using ion trap electrospray ionization mass spectrometry to classify ligands to a target molecule in terms of kinetic parameters. We demonstrate this method in the screening of ligands to a hexahistidine tag from a pooled library synthesized by click chemistry. The ion trap mass spectrometry analysis revealed that higher stabilities of ligand-target complexes in the gas phase were related to lower dissociation rate constants, i.e., off-rates in solution. Finally, we prepared a fluorescent probe utilizing the ligand with lowest off-rate and succeeded in performing single molecule observations of hexahistidine-tagged myosin V walking on actin filaments.

本文言語English
ページ(範囲)451-455
ページ数5
ジャーナルACS Combinatorial Science
14
8
DOI
出版ステータスPublished - 2012 8 13

ASJC Scopus subject areas

  • Chemistry(all)

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