Maturation of the extracellular matrix and cell adhesion molecules in layered co-cultures of HepG2 and endothelial cells

Maki Ohno, Kiyoto Motojima, Teruo Okano, Akiyoshi Taniguchi*

*この研究の対応する著者

研究成果: Article査読

27 被引用数 (Scopus)

抄録

We previously reported that using thermo-responsive culture surfaces, a layered co-culture was achieved by placing an endothelial cell sheet onto a layer of human hepatoma cell line HepG2 in order to develop a culture model that mimics hepatic lobules. In the layered co-culture cells, the expression levels of liver-specific genes gradually increased. A cross-sectional view of the layered co-culture cells showed that the thickness of the layer slowly increased after layering, as did extracellular matrix (ECM) deposition around HepG2 cells. In this study, we report that the molecular compositions of ECM and cell-adhesion molecules changed in the layered co-culture cells. Gene expression of integrin α4 and decorin gradually increased after layering, and the time-course pattern of these genes was correlated with that of liver-specific genes. Moreover, the layered co-culture system has the ability to assemble a branching network of fibronectin fibrils. These results suggest that a vastly different extracellular environment in layered co-culture cells may induce an increase in liver-specific functions.

本文言語English
ページ(範囲)591-597
ページ数7
ジャーナルJournal of biochemistry
145
5
DOI
出版ステータスPublished - 2009 5月 1
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 分子生物学

フィンガープリント

「Maturation of the extracellular matrix and cell adhesion molecules in layered co-cultures of HepG2 and endothelial cells」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル