MazF endoribonucleolytic toxin conserved in nitrospira specifically cleaves the AACU, AACG, and AAUU motifs

Rie Aoi, Tatsuki Miyamoto, Akiko Yokota, Yuri Ota, Hirotsugu Fujitani, Satoshi Tsuneda, Naohiro Noda

研究成果: Article査読

1 被引用数 (Scopus)

抄録

MazF is an endoribonucleolytic toxin that cleaves intracellular RNAs in sequence-specific manners. It is liberated in bacterial cells in response to environmental changes and is suggested to contribute to bacterial survival by inducing translational regulation. Thus, determining the cleavage specificity provides insights into the physiological functions of MazF orthologues. Nitrospira, detected in a wide range of environments, is thought to have evolved the ability to cope with their surroundings. To investigate the molecular mechanism of its environmental adaption, a MazF module from Nitrospira strain ND1, which was isolated from the activated sludge of a wastewater treatment plant, is examined in this study. By combining a massive parallel sequencing method and fluorometric assay, we detected that this functional RNA-cleaving toxin specifically recognizes the AACU, AACG, and AAUU motifs. Additionally, statistical analysis suggested that this enzyme regulates various specific functions in order to resist environmental stresses.

本文言語English
論文番号287
ジャーナルToxins
12
5
DOI
出版ステータスPublished - 2020 5

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

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