Measurement of enzymatic and motile activities of Arabidopsis myosins by using Arabidopsis actins

Sa Rula, Takahiro Suwa, Saku T. Kijima, Takeshi Haraguchi, Shinryu Wakatsuki, Naruki Sato, Zhongrui Duan, Motoki Tominaga, Taro Q.P. Uyeda, Kohji Ito

研究成果: Article

2 引用 (Scopus)

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There are two classes of myosin, XI and VIII, in higher plants. Myosin XI moves actin filaments at high speed and its enzyme activity is also very high. In contrast, myosin VIII moves actin filaments very slowly with very low enzyme activity. Because most of these enzymatic and motile activities were measured using animal skeletal muscle α-actin, but not plant actin, they would not accurately reflect the actual activities in plant cells. We thus measured enzymatic and motile activities of the motor domains of two Arabidopsis myosin XI isoforms (MYA2, XI-B), and one Arabidopsis myosin VIII isoform (ATM1), by using three Arabidopsis actin isoforms (ACT1, ACT2, and ACT7). The measured activities were different from those measured by using muscle actin. Moreover, Arabidopsis myosins showed different enzymatic and motile activities when using different Arabidopsis actin isoforms. Our results suggest that plant actin should be used for measuring enzymatic and motile activities of plant myosins and that different actin isoforms in plant cells might function as different tracks along which affinities and velocities of each myosin isoform are modulated.

元の言語English
ページ(範囲)2145-2151
ページ数7
ジャーナルBiochemical and Biophysical Research Communications
495
発行部数3
DOI
出版物ステータスPublished - 2018 1 15

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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