TY - GEN
T1 - Measurement of inflammatory cytokine secretion from human monocytes after inflammasome activation
AU - Shirasaki, Yoshitaka
AU - Nakahara, Asahi
AU - Shimura, Nanako
AU - Izawa, Kazushi
AU - Suzuki, Nobutake
AU - Yamagishi, Mai
AU - Mizuno, Jun
AU - Sekiguchi, Tetsushi
AU - Nishikomori, Ryuta
AU - Shoji, Shuichi
AU - Ohara, Osamu
PY - 2012/1/1
Y1 - 2012/1/1
N2 - Live-cell imaging of inflammatory cytokine secretion from human monocytes was successfully performed on a microfabricated well array chip by time-resolved fluoroimmunoassay using total internal reflection fluorescence microscopy. Some of the lipopolysaccharide primed monocytes secreted small amounts of interleukin-1 beta (IL-1 ), whereas the monocytes stimulated with an ATP signal secreted large amounts of IL-1 from 10% of observed cells. These two types of secretion patterns corresponded to the proposed models of IL-1 secretion, "rescue and redirect" and "terminal release", respectively. Morphological changes in ATP-stimulated cells also indicated that the cells underwent necrotic cell death, also known as "pyroptosis".
AB - Live-cell imaging of inflammatory cytokine secretion from human monocytes was successfully performed on a microfabricated well array chip by time-resolved fluoroimmunoassay using total internal reflection fluorescence microscopy. Some of the lipopolysaccharide primed monocytes secreted small amounts of interleukin-1 beta (IL-1 ), whereas the monocytes stimulated with an ATP signal secreted large amounts of IL-1 from 10% of observed cells. These two types of secretion patterns corresponded to the proposed models of IL-1 secretion, "rescue and redirect" and "terminal release", respectively. Morphological changes in ATP-stimulated cells also indicated that the cells underwent necrotic cell death, also known as "pyroptosis".
KW - Cytokine secretion
KW - Inflammation
KW - Monocyte
KW - Single cell
UR - http://www.scopus.com/inward/record.url?scp=84901797416&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84901797416&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84901797416
SN - 9780979806452
T3 - Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
SP - 1012
EP - 1014
BT - Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PB - Chemical and Biological Microsystems Society
T2 - 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
Y2 - 28 October 2012 through 1 November 2012
ER -