Measuring the vascular diameter of brain surface and parenchymal arteries in awake mouse

Yuta Sekiguchi, Kazuto Masamoto, Hiroyuki Takuwa, Hiroshi Kawaguchi, Iwao Kanno, Hiroshi Ito, Yutaka Tomita, Yoshiaki Itoh, Norihiro Suzuki, Ryo Sudo, Kazuo Tanishita

研究成果: Conference contribution

抄録

The present study reports a semiautomatic image analysis method for measuring the spatiotemporal dynamics of the vessel dilation that was fluorescently imaged with either confocal or two-photon microscope. With this method, arterial dilation induced by whisker stimulation was compared between cortical surface and parenchymal tissue in the vibrissae area of somatosensory cortex in awake Tie2-GFP mice in which the vascular endothelium had genetically expressed green fluorescent protein. We observed that a mean arterial diameter during a pre-stimulus baseline state was 39 ± 7, 19 ± 1, 16 ± 4, 17 ± 4, and 14 ± 3 μm at depths of 0, 100, 200, 300, and 400 μm, respectively. The stimulation-evoked dilation induced by mechanical whisker deflection (10 Hz for 5 s) was 3.4 ± 0.8, 1.8 ± 0.8, 1.8 ± 0.9, 1.6 ± 0.9, and 1.5 ± 0.6 μm at each depth, respectively. Consequently, no significant differences were observed for the vessel dilation rate between the cortical surface and parenchymal arteries: 8.8 %, 9.9 %, 10.9 %, 9.2 %, and 10.3 % relative to their baseline diameters, respectively. These preliminary results demonstrate that the present method is useful to further investigate the quantitative relationships between the spatiotemporally varying arterial tone and the associated blood flow changes in the parenchymal microcirculation to reveal the regulatory mechanism of the cerebral blood flow.

元の言語English
ホスト出版物のタイトルAdvances in Experimental Medicine and Biology
ページ419-425
ページ数7
789
DOI
出版物ステータスPublished - 2013
外部発表Yes

出版物シリーズ

名前Advances in Experimental Medicine and Biology
789
ISSN(印刷物)00652598

Fingerprint

Vibrissae
Blood Vessels
Dilatation
Brain
Blood
Arteries
Microcirculation
Green Fluorescent Proteins
Image analysis
Cerebrovascular Circulation
Microscopes
Photons
Tissue
Somatosensory Cortex
Vascular Endothelium

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

これを引用

Sekiguchi, Y., Masamoto, K., Takuwa, H., Kawaguchi, H., Kanno, I., Ito, H., ... Tanishita, K. (2013). Measuring the vascular diameter of brain surface and parenchymal arteries in awake mouse. : Advances in Experimental Medicine and Biology (巻 789, pp. 419-425). (Advances in Experimental Medicine and Biology; 巻数 789). https://doi.org/10.1007/978-1-4614-7411-1-56

Measuring the vascular diameter of brain surface and parenchymal arteries in awake mouse. / Sekiguchi, Yuta; Masamoto, Kazuto; Takuwa, Hiroyuki; Kawaguchi, Hiroshi; Kanno, Iwao; Ito, Hiroshi; Tomita, Yutaka; Itoh, Yoshiaki; Suzuki, Norihiro; Sudo, Ryo; Tanishita, Kazuo.

Advances in Experimental Medicine and Biology. 巻 789 2013. p. 419-425 (Advances in Experimental Medicine and Biology; 巻 789).

研究成果: Conference contribution

Sekiguchi, Y, Masamoto, K, Takuwa, H, Kawaguchi, H, Kanno, I, Ito, H, Tomita, Y, Itoh, Y, Suzuki, N, Sudo, R & Tanishita, K 2013, Measuring the vascular diameter of brain surface and parenchymal arteries in awake mouse. : Advances in Experimental Medicine and Biology. 巻. 789, Advances in Experimental Medicine and Biology, 巻. 789, pp. 419-425. https://doi.org/10.1007/978-1-4614-7411-1-56
Sekiguchi Y, Masamoto K, Takuwa H, Kawaguchi H, Kanno I, Ito H その他. Measuring the vascular diameter of brain surface and parenchymal arteries in awake mouse. : Advances in Experimental Medicine and Biology. 巻 789. 2013. p. 419-425. (Advances in Experimental Medicine and Biology). https://doi.org/10.1007/978-1-4614-7411-1-56
Sekiguchi, Yuta ; Masamoto, Kazuto ; Takuwa, Hiroyuki ; Kawaguchi, Hiroshi ; Kanno, Iwao ; Ito, Hiroshi ; Tomita, Yutaka ; Itoh, Yoshiaki ; Suzuki, Norihiro ; Sudo, Ryo ; Tanishita, Kazuo. / Measuring the vascular diameter of brain surface and parenchymal arteries in awake mouse. Advances in Experimental Medicine and Biology. 巻 789 2013. pp. 419-425 (Advances in Experimental Medicine and Biology).
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