Mechanical properties of membrane surface of cultured astrocyte revealed by atomic force microscopy

Hatsuki Shiga*, Yukako Yamane, Etsuro Ito, Kazuhiro Abe, Kazushige Kawabata, Hisashi Haga

*この研究の対応する著者

研究成果: Article査読

16 被引用数 (Scopus)

抄録

In order to examine the mechanical properties of the membrane surface of astrocytes, we observed living astrocytes by atomic force microscopy (AFM) both in contact mode and force-mapping mode. Ridge-like structures reflecting actin filaments were observed in the topographic images in contact mode, but not in force-mapping mode, using a zero-loading force. When we measured the elasticity of astrocytes, we observed that the cell membrane above the nucleus was soft and the cell membrane above the cytosol was stiff. In particular, the parts reflecting actin filaments were very stiff. This effect of actin filaments on the elasticity of astrocytes was confirmed by the loss of actin filaments after application of actin-polymerization inhibitor.

本文言語English
ページ(範囲)3711-3716
ページ数6
ジャーナルJapanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers
39
6 B
DOI
出版ステータスPublished - 2000
外部発表はい

ASJC Scopus subject areas

  • 工学(全般)
  • 物理学および天文学(全般)

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