Mechanical properties of membrane surface of cultured astrocyte revealed by atomic force microscopy

Hatsuki Shiga, Yukako Yamane, Etsuro Ito, Kazuhiro Abe, Kazushige Kawabata, Hisashi Haga

研究成果: Article

15 引用 (Scopus)

抜粋

In order to examine the mechanical properties of the membrane surface of astrocytes, we observed living astrocytes by atomic force microscopy (AFM) both in contact mode and force-mapping mode. Ridge-like structures reflecting actin filaments were observed in the topographic images in contact mode, but not in force-mapping mode, using a zero-loading force. When we measured the elasticity of astrocytes, we observed that the cell membrane above the nucleus was soft and the cell membrane above the cytosol was stiff. In particular, the parts reflecting actin filaments were very stiff. This effect of actin filaments on the elasticity of astrocytes was confirmed by the loss of actin filaments after application of actin-polymerization inhibitor.

元の言語English
ページ(範囲)3711-3716
ページ数6
ジャーナルJapanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers
39
発行部数6 B
出版物ステータスPublished - 2000 12 1

ASJC Scopus subject areas

  • Engineering(all)
  • Physics and Astronomy(all)

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