Mechanochemical coupling of two substeps in a single myosin V motor

Sotaro Uemura, Hideo Higuchi, Adrian O. Olivares, Enrique M. De La Cruz, Shin'ichi Ishiwata*

*この研究の対応する著者

    研究成果: Article査読

    151 被引用数 (Scopus)

    抄録

    Myosin V is a double-headed processive molecular motor that moves along an actin filament by taking 36-nm steps. Using optical trapping nanometry with high spatiotemporal resolution, we discovered that there are two possible pathways for the 36-nm steps, one with 12- and 24-nm substeps, in this order, and the other without substeps. Based on the analyses of effects of ATP, ADP and 2,3-butanedione 2-monoxime (a reagent shown here to slow ADP release from actomyosin V) on the dwell time and the occurrence frequency of the main and the intermediate states, we propose that the 12-nm substep occurs after ATP binding to the bound trailing head and the 24-nm substep results from a mechanical step following the isomerization of an actomyosin-ADP state on the bound leading head. When the isomerization precedes the 12-nm substep, the 36-nm step occurs without substeps.

    本文言語English
    ページ(範囲)877-883
    ページ数7
    ジャーナルNature Structural and Molecular Biology
    11
    9
    DOI
    出版ステータスPublished - 2004 9月

    ASJC Scopus subject areas

    • 構造生物学
    • 分子生物学

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