Megakaryocytes derived from CD34-positive cord blood cells produce interleukin-8

Tsukasa Higuchi, Kenichi Koike*, Nobukuni Sawai, Hadija Hemed Mwamtemi, Kouichi Takeuchi, Masaaki Shiohara, Toshimi Kikuchi, Kozo Yasui, Susumu Ito, Osamu Yamagami, Yumiko Sasaki, Nobuo Okumura, Takashi Kato, Hiroshi Miyazaki, Masayuki Ikeda, Muneo Yamada, Atsushi Komiyama

*この研究の対応する著者

研究成果: Article査読

14 被引用数 (Scopus)

抄録

In a serum-free liquid culture, thrombopoietin (TPD) selectively stimulated the growth of megakaryocytic cells from CD34-positive cord blood cells. Using these cultured cells, we investigated cytokine production by human megakaryocytes. Day 10 megakaryocytes (2 x 105) secreted > 1000 pg/ml of interleukin (IL)-8, in contrast to small amounts of IL-1β and IL-6. A time-course study showed that the IL-8 production of megakaryocytes occurred at the late phase of the culture period. The megakaryocyte-conditioned medium had the chemotactic potential of polymorphonuclear leucocytes, which was abrogated by the addition of anti-IL-8 antibody, suggesting the secretion of biologically active IL-8. The combination of TPO and IL-1α was required for a significant augmentation of the IL-8 secretion. Direct evidence for IL-8 synthesis in megakaryocytes was provided by reverse transcription-polymerase chain reaction on purified CD41b+ cells and by the detection of intracellular IL-8 in CD41b+ cells. These results suggest that TPO stimulates not only the proliferation and differentiation of the progenitors capable of megakaryocytic lineage expression but also IL-8 release by the megakaryocytic cells with the aid of IL-1.

本文言語English
ページ(範囲)509-516
ページ数8
ジャーナルBritish Journal of Haematology
99
3
DOI
出版ステータスPublished - 1997
外部発表はい

ASJC Scopus subject areas

  • 血液学

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