Metabolomic profiling analysis reveals chamber-dependent metabolite patterns in the mouse heart

Daisuke Shimura, Gaku Nakai, Qibin Jiao, Kota Osanai, Kasumi Kashikura, Keiko Endo, Tomoyoshi Soga, Nobuhito Goda, Susumu Minamisawa

    研究成果: Article

    11 引用 (Scopus)

    抄録

    Energy of the cardiac muscle largely depends on fatty acid oxidation. It is known that the atrium and ventricle have chamber-specific functions, structures, gene expressions, and pathologies. The left ventricle works as a high-pressure chamber to pump blood toward the body, and its muscle wall is thicker than those of the other chambers, suggesting that energy utilization in each of the chambers should be different. However, a chamber-specific pattern of metabolism remains incompletely understood. Recently, innovative techniques have enabled the comprehensive analysis of metabolites. Therefore, we aimed to clarify differences in metabolic patterns among the chambers. Male C57BL6 mice at 6 wk old were subject to a comprehensive measurement of metabolites in the atria and ventricles by capillary electrophoresis and mass spectrometry. We found that overall metabolic profiles, including nucleotides and amino acids, were similar between the right and left ventricles. On the other hand, the atria exhibited a distinct metabolic pattern from those of the ventricles. Importantly, the highenergy phosphate pool (the total concentration of ATP, ADP, and AMP) was higher in both ventricles. In addition, the levels of lactate, acetyl CoA, and tricarboxylic acid cycle contents were higher in the ventricles. Accordingly, the activities and/or expression levels of key enzymes were higher in the ventricles to produce more energy. The present study provides a basis for understanding the chamber-specific metabolism underlining pathophysiology in the heart.

    元の言語English
    ジャーナルAmerican Journal of Physiology - Heart and Circulatory Physiology
    305
    発行部数4
    DOI
    出版物ステータスPublished - 2013 5 8

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    Metabolomics
    Heart Ventricles
    Acetyl Coenzyme A
    Citric Acid Cycle
    Metabolome
    Capillary Electrophoresis
    Adenosine Monophosphate
    Adenosine Diphosphate
    Lactic Acid
    Mass Spectrometry
    Myocardium
    Fatty Acids
    Nucleotides
    Adenosine Triphosphate
    Phosphates
    Pathology
    Gene Expression
    Amino Acids
    Pressure
    Muscles

    ASJC Scopus subject areas

    • Physiology
    • Physiology (medical)
    • Cardiology and Cardiovascular Medicine

    これを引用

    Metabolomic profiling analysis reveals chamber-dependent metabolite patterns in the mouse heart. / Shimura, Daisuke; Nakai, Gaku; Jiao, Qibin; Osanai, Kota; Kashikura, Kasumi; Endo, Keiko; Soga, Tomoyoshi; Goda, Nobuhito; Minamisawa, Susumu.

    :: American Journal of Physiology - Heart and Circulatory Physiology, 巻 305, 番号 4, 08.05.2013.

    研究成果: Article

    Shimura, Daisuke ; Nakai, Gaku ; Jiao, Qibin ; Osanai, Kota ; Kashikura, Kasumi ; Endo, Keiko ; Soga, Tomoyoshi ; Goda, Nobuhito ; Minamisawa, Susumu. / Metabolomic profiling analysis reveals chamber-dependent metabolite patterns in the mouse heart. :: American Journal of Physiology - Heart and Circulatory Physiology. 2013 ; 巻 305, 番号 4.
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    abstract = "Energy of the cardiac muscle largely depends on fatty acid oxidation. It is known that the atrium and ventricle have chamber-specific functions, structures, gene expressions, and pathologies. The left ventricle works as a high-pressure chamber to pump blood toward the body, and its muscle wall is thicker than those of the other chambers, suggesting that energy utilization in each of the chambers should be different. However, a chamber-specific pattern of metabolism remains incompletely understood. Recently, innovative techniques have enabled the comprehensive analysis of metabolites. Therefore, we aimed to clarify differences in metabolic patterns among the chambers. Male C57BL6 mice at 6 wk old were subject to a comprehensive measurement of metabolites in the atria and ventricles by capillary electrophoresis and mass spectrometry. We found that overall metabolic profiles, including nucleotides and amino acids, were similar between the right and left ventricles. On the other hand, the atria exhibited a distinct metabolic pattern from those of the ventricles. Importantly, the highenergy phosphate pool (the total concentration of ATP, ADP, and AMP) was higher in both ventricles. In addition, the levels of lactate, acetyl CoA, and tricarboxylic acid cycle contents were higher in the ventricles. Accordingly, the activities and/or expression levels of key enzymes were higher in the ventricles to produce more energy. The present study provides a basis for understanding the chamber-specific metabolism underlining pathophysiology in the heart.",
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