Methodological improvements of pyrosequencing technology

Baback Gharizadeh, Michael Akhras, Nader Nourizad, Mehran Ghaderi, Kenji Yasuda, Pål Nyrén, Nader Pourmand

研究成果: Article

25 引用 (Scopus)

抄録

Pyrosequencing technology is a rather novel DNA sequencing method based on the sequencing-by-synthesis principle. This bioluminometric, real-time DNA sequencing technique employs a cascade of four enzymatic reactions producing sequence peak signals. The method has been proven highly suitable for single nucleotide polymorphism analysis and sequencing of short stretches of DNA. Although the pyrosequencing procedure is relatively straightforward, users may face challenges due to varying parameters in PCR and sequencing primer design, sample preparation and nucleotide dispensation; such challenges are labor and cost intensive. In this study, these issues have been addressed to increase signal quality and assure sequence accuracy.

元の言語English
ページ(範囲)504-511
ページ数8
ジャーナルJournal of Biotechnology
124
発行部数3
DOI
出版物ステータスPublished - 2006 7 25
外部発表Yes

Fingerprint

DNA
Nucleotides
Technology
DNA Sequence Analysis
Protein Sorting Signals
Polymorphism
Single Nucleotide Polymorphism
Personnel
Costs and Cost Analysis
Polymerase Chain Reaction
Costs

ASJC Scopus subject areas

  • Biotechnology

これを引用

Gharizadeh, B., Akhras, M., Nourizad, N., Ghaderi, M., Yasuda, K., Nyrén, P., & Pourmand, N. (2006). Methodological improvements of pyrosequencing technology. Journal of Biotechnology, 124(3), 504-511. https://doi.org/10.1016/j.jbiotec.2006.01.025

Methodological improvements of pyrosequencing technology. / Gharizadeh, Baback; Akhras, Michael; Nourizad, Nader; Ghaderi, Mehran; Yasuda, Kenji; Nyrén, Pål; Pourmand, Nader.

:: Journal of Biotechnology, 巻 124, 番号 3, 25.07.2006, p. 504-511.

研究成果: Article

Gharizadeh, B, Akhras, M, Nourizad, N, Ghaderi, M, Yasuda, K, Nyrén, P & Pourmand, N 2006, 'Methodological improvements of pyrosequencing technology', Journal of Biotechnology, 巻. 124, 番号 3, pp. 504-511. https://doi.org/10.1016/j.jbiotec.2006.01.025
Gharizadeh B, Akhras M, Nourizad N, Ghaderi M, Yasuda K, Nyrén P その他. Methodological improvements of pyrosequencing technology. Journal of Biotechnology. 2006 7 25;124(3):504-511. https://doi.org/10.1016/j.jbiotec.2006.01.025
Gharizadeh, Baback ; Akhras, Michael ; Nourizad, Nader ; Ghaderi, Mehran ; Yasuda, Kenji ; Nyrén, Pål ; Pourmand, Nader. / Methodological improvements of pyrosequencing technology. :: Journal of Biotechnology. 2006 ; 巻 124, 番号 3. pp. 504-511.
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