Micro-vessel network formation of endothelial cells with in vitro three dimensional model

K. Tanishita, A. Ueda, M. Koga, R. Sudo, S. Kudo, M. Ikeda

研究成果: Chapter

抄録

Bovine pulmonary microvascular endothelial cells were seeded onto collagen gels with basic fibroblast growth factor (bFGF) to make a micro-vessel formation model. We observed this model in detail using phase contrast microscopy, confocal laser scanning microscopy and electron microscopy. The results show that cells invaded the collagen gel and reconstructed the tubular structures, containing a clearly defined lumen consisting of multiple cells. The model was placed in a parallel-plate flow chamber. A laminar shear stress of 0.3 Pa was applied to the surfaces of the cells for 48 hours. Promotion of micro-vessel network formation was detectable after approximately 10 hours in the flow chamber. After 48 hours, the length of networks exposed to shear stress was 6.17 (± 0.59) times longer than at the initial state, whereas the length of networks not exposed to shear stress was only 3.30 (± 0.41) times longer. The number of bifurcations and endpoints increased for networks exposed to shear stress, whereas the number of bifurcations alone increased for networks not exposed to shear stress. These results demonstrate that shear stress applied to the surfaces of endothelial cells on collagen gel promotes the growth of micro-vessel network formation in the gel and expands the network due to repeated bifurcation and elongation.

元の言語English
ホスト出版物のタイトルBiomechanics at Micro- and Nanoscale Levels
出版者World Scientific Publishing Co.
ページ26-36
ページ数11
2
ISBN(印刷物)9789812773838, 9812567461, 9789812567468
DOI
出版物ステータスPublished - 2006 1 1
外部発表Yes

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Endothelial cells
Shear stress
Endothelial Cells
Gels
Collagen
Phase-Contrast Microscopy
Fibroblast Growth Factor 2
Confocal Microscopy
Electron Microscopy
Confocal microscopy
Lung
Electron microscopy
In Vitro Techniques
Elongation
Microscopic examination
Growth
Scanning
Lasers

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

これを引用

Tanishita, K., Ueda, A., Koga, M., Sudo, R., Kudo, S., & Ikeda, M. (2006). Micro-vessel network formation of endothelial cells with in vitro three dimensional model. : Biomechanics at Micro- and Nanoscale Levels (巻 2, pp. 26-36). World Scientific Publishing Co.. https://doi.org/10.1142/9789812773838_0003

Micro-vessel network formation of endothelial cells with in vitro three dimensional model. / Tanishita, K.; Ueda, A.; Koga, M.; Sudo, R.; Kudo, S.; Ikeda, M.

Biomechanics at Micro- and Nanoscale Levels. 巻 2 World Scientific Publishing Co., 2006. p. 26-36.

研究成果: Chapter

Tanishita, K, Ueda, A, Koga, M, Sudo, R, Kudo, S & Ikeda, M 2006, Micro-vessel network formation of endothelial cells with in vitro three dimensional model. : Biomechanics at Micro- and Nanoscale Levels. 巻. 2, World Scientific Publishing Co., pp. 26-36. https://doi.org/10.1142/9789812773838_0003
Tanishita K, Ueda A, Koga M, Sudo R, Kudo S, Ikeda M. Micro-vessel network formation of endothelial cells with in vitro three dimensional model. : Biomechanics at Micro- and Nanoscale Levels. 巻 2. World Scientific Publishing Co. 2006. p. 26-36 https://doi.org/10.1142/9789812773838_0003
Tanishita, K. ; Ueda, A. ; Koga, M. ; Sudo, R. ; Kudo, S. ; Ikeda, M. / Micro-vessel network formation of endothelial cells with in vitro three dimensional model. Biomechanics at Micro- and Nanoscale Levels. 巻 2 World Scientific Publishing Co., 2006. pp. 26-36
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