Microbial community structure in autotrophic nitrifying granules characterized by experimental and simulation analyses

Shinya Matsumoto, Mayu Katoku, Goro Saeki, Akihiko Terada, Yoshiteru Aoi, Satoshi Tsuneda, Cristian Picioreanu, Mark C M Van Loosdrecht

    研究成果: Article

    84 引用 (Scopus)

    抄録

    This study evaluates the community structure in nitrifying granules (average diameter of 1600 μm) produced in an aerobic reactor fed with ammonia as the sole energy source by a multivalent approach combining molecular techniques, microelectrode measurements and mathematical modelling. Fluorescence in situ hybridization revealed that ammonia-oxidizing bacteria dominated within the first 200 μm below the granule surface, nitrite-oxidizing bacteria a deeper layer between 200 and 300 μm, while heterotrophic bacteria were present in the core of the nitrifying granule. Presence of these groups also became evident from a 16S rRNA clone library. Microprofiles of NH 4 +, NO2 -, NO3 - and O2 concentrations measured with microelectrodes showed good agreement with the spatial organization of nitrifying bacteria. One- and two-dimensional numerical biofilm models were constructed to explain the observed granule development as a result of the multiple bacteria-substrate interactions. The interaction between nitrifying and heterotrophic bacteria was evaluated by assuming three types of heterotrophic bacterial growth on soluble microbial products from nitrifying bacteria. The models described well the bacterial distribution obtained by fluorescence in situ hybridization analysis, as well as the measured oxygen, nitrite, nitrate and ammonium concentration profiles. Results of this study are important because they show that a combination of simulation and experimental techniques can better explain the interaction between nitrifying bacteria and heterotrophic bacteria in the granules than individual approaches alone.

    元の言語English
    ページ(範囲)192-206
    ページ数15
    ジャーナルEnvironmental Microbiology
    12
    発行部数1
    DOI
    出版物ステータスPublished - 2010 1

    Fingerprint

    microbial communities
    microbial community
    granules
    community structure
    Bacteria
    nitrifying bacterium
    bacterium
    bacteria
    simulation
    fluorescence in situ hybridization
    nitrites
    nitrite
    fluorescence
    ammonia
    Microelectrodes
    Nitrites
    Fluorescence In Situ Hybridization
    Ammonia
    Heterotrophic Processes
    biofilm

    ASJC Scopus subject areas

    • Microbiology
    • Ecology, Evolution, Behavior and Systematics

    これを引用

    Microbial community structure in autotrophic nitrifying granules characterized by experimental and simulation analyses. / Matsumoto, Shinya; Katoku, Mayu; Saeki, Goro; Terada, Akihiko; Aoi, Yoshiteru; Tsuneda, Satoshi; Picioreanu, Cristian; Van Loosdrecht, Mark C M.

    :: Environmental Microbiology, 巻 12, 番号 1, 01.2010, p. 192-206.

    研究成果: Article

    Matsumoto, Shinya ; Katoku, Mayu ; Saeki, Goro ; Terada, Akihiko ; Aoi, Yoshiteru ; Tsuneda, Satoshi ; Picioreanu, Cristian ; Van Loosdrecht, Mark C M. / Microbial community structure in autotrophic nitrifying granules characterized by experimental and simulation analyses. :: Environmental Microbiology. 2010 ; 巻 12, 番号 1. pp. 192-206.
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    abstract = "This study evaluates the community structure in nitrifying granules (average diameter of 1600 μm) produced in an aerobic reactor fed with ammonia as the sole energy source by a multivalent approach combining molecular techniques, microelectrode measurements and mathematical modelling. Fluorescence in situ hybridization revealed that ammonia-oxidizing bacteria dominated within the first 200 μm below the granule surface, nitrite-oxidizing bacteria a deeper layer between 200 and 300 μm, while heterotrophic bacteria were present in the core of the nitrifying granule. Presence of these groups also became evident from a 16S rRNA clone library. Microprofiles of NH 4 +, NO2 -, NO3 - and O2 concentrations measured with microelectrodes showed good agreement with the spatial organization of nitrifying bacteria. One- and two-dimensional numerical biofilm models were constructed to explain the observed granule development as a result of the multiple bacteria-substrate interactions. The interaction between nitrifying and heterotrophic bacteria was evaluated by assuming three types of heterotrophic bacterial growth on soluble microbial products from nitrifying bacteria. The models described well the bacterial distribution obtained by fluorescence in situ hybridization analysis, as well as the measured oxygen, nitrite, nitrate and ammonium concentration profiles. Results of this study are important because they show that a combination of simulation and experimental techniques can better explain the interaction between nitrifying bacteria and heterotrophic bacteria in the granules than individual approaches alone.",
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