Microbial community structure in autotrophic nitrifying granules characterized by experimental and simulation analyses

Shinya Matsumoto, Mayu Katoku, Goro Saeki, Akihiko Terada, Yoshiteru Aoi, Satoshi Tsuneda*, Cristian Picioreanu, Mark C.M. Van Loosdrecht

*この研究の対応する著者

研究成果: Article査読

114 被引用数 (Scopus)

抄録

This study evaluates the community structure in nitrifying granules (average diameter of 1600 μm) produced in an aerobic reactor fed with ammonia as the sole energy source by a multivalent approach combining molecular techniques, microelectrode measurements and mathematical modelling. Fluorescence in situ hybridization revealed that ammonia-oxidizing bacteria dominated within the first 200 μm below the granule surface, nitrite-oxidizing bacteria a deeper layer between 200 and 300 μm, while heterotrophic bacteria were present in the core of the nitrifying granule. Presence of these groups also became evident from a 16S rRNA clone library. Microprofiles of NH 4+, NO2-, NO3- and O2 concentrations measured with microelectrodes showed good agreement with the spatial organization of nitrifying bacteria. One- and two-dimensional numerical biofilm models were constructed to explain the observed granule development as a result of the multiple bacteria-substrate interactions. The interaction between nitrifying and heterotrophic bacteria was evaluated by assuming three types of heterotrophic bacterial growth on soluble microbial products from nitrifying bacteria. The models described well the bacterial distribution obtained by fluorescence in situ hybridization analysis, as well as the measured oxygen, nitrite, nitrate and ammonium concentration profiles. Results of this study are important because they show that a combination of simulation and experimental techniques can better explain the interaction between nitrifying bacteria and heterotrophic bacteria in the granules than individual approaches alone.

本文言語English
ページ(範囲)192-206
ページ数15
ジャーナルEnvironmental Microbiology
12
1
DOI
出版ステータスPublished - 2010 1月

ASJC Scopus subject areas

  • 微生物学
  • 生態、進化、行動および分類学

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