Microbial population dynamics and community structure during the formation of nitrifying granules to treat ammonia-rich inorganic wastewater

Shinya Matsumoto, Daisuke Ishikawa, Goro Saeki, Yoshiteru Aoi, Satoshi Tsuneda

    研究成果: Article

    15 引用 (Scopus)

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    Microbial population dynamics were investigated during the formation of nitrifying granules in an aerobic upflow fluidized bed (AUFB) reactor fed ammonia as a sole energy source. Analyses of clone libraries of 16S rRNA gene and the ammonia monooxygenase subunit A gene (amoA) revealed that although the clones obtained from the seed sludge were widely distributed among the ammonia-oxidizing bacteria (AOB) isolates, the community structure of AOB shifted towards the Nitrosomonas mobilis lineage as granulation proceeded. Quantitative fluorescence in situ hybridization showed that changes in the bacterial population occurred concomitantly with changes in nitrification performance and the size of granules. AOB associated with the N. mobilis lineage were predominant in the early stages as nitrifying granules formed (average diameter, 126 μm). In mature granules (average diameter, 270 μm), at least three types of AOB, N. mobilis, Nitrosomonas oligotropha, and Nitrosomonas europaea, formed different niches and coexisted. Nitrite-oxidizing bacteria (NOB) affiliated with Nitrospira spp. were detected in the start-up period, but were replaced by NOB affiliated with Nitrobacter spp. after granules formed.

    元の言語English
    ページ(範囲)164-170
    ページ数7
    ジャーナルMicrobes and Environments
    25
    発行部数3
    DOI
    出版物ステータスPublished - 2010

    Fingerprint

    wastewater
    granules
    population dynamics
    community structure
    ammonia
    bacterium
    bacteria
    nitrites
    nitrite
    Nitrosomonas oligotropha
    clone
    Nitrobacter
    Nitrosomonas europaea
    Nitrosomonas
    RNA libraries
    clones
    fluidized beds
    gene
    fluorescence in situ hybridization
    sludge

    ASJC Scopus subject areas

    • Plant Science
    • Soil Science
    • Ecology, Evolution, Behavior and Systematics

    これを引用

    Microbial population dynamics and community structure during the formation of nitrifying granules to treat ammonia-rich inorganic wastewater. / Matsumoto, Shinya; Ishikawa, Daisuke; Saeki, Goro; Aoi, Yoshiteru; Tsuneda, Satoshi.

    :: Microbes and Environments, 巻 25, 番号 3, 2010, p. 164-170.

    研究成果: Article

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    AU - Aoi, Yoshiteru

    AU - Tsuneda, Satoshi

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