Microbial populations responsive to denitrification-inducing conditions in rice paddy soil, as revealed by comparative 16S rRNA gene analysis

Satoshi Ishii, Michihiro Yamamoto, Mami Kikuchi, Kenshiro Oshima, Masahira Hattori, Shigeto Otsuka, Keishi Senoo

研究成果: Article査読

75 被引用数 (Scopus)

抄録

Rice paddy soil has been shown to have strong denitrifying activity. However, the microbial populations responsible for nitrate respiration and denitrification have not been well characterized. In this study, we performed a clone library analysis of >1,000 clones of the nearly full-length 16S rRNA gene to characterize bacterial community structure in rice paddy soil. We also identified potential key players in nitrate respiration and denitrification by comparing the community structures of soils with strong denitrifying activity to those of soils without denitrifying activity. Clone library analysis showed that bacteria belonging to the phylum Firmicutes, including a unique Symbiobacterium clade, dominated the clones obtained in this study. Using the template match method, several operational taxonomic units (OTUs), most belonging to the orders Burkholderiales and Rhodocyclales, were identified as OTUs that were specifically enriched in the sample with strong denitrifying activity. Almost one-half of these OTUs were classified in the genus Herbaspirillum and appeared >10-fold more frequently in the soils with strong denitrifying activity than in the soils without denitrifying activity. Therefore, OTUs related to Herbaspirillum are potential key players in nitrate respiration and denitrification under the conditions used.

本文言語English
ページ(範囲)7070-7078
ページ数9
ジャーナルApplied and Environmental Microbiology
75
22
DOI
出版ステータスPublished - 2009 11
外部発表はい

ASJC Scopus subject areas

  • 応用微生物学とバイオテクノロジー
  • 食品科学
  • バイオテクノロジー
  • 生態学

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