Microfabricated on-chip-type electrochemical flow immunoassay system for the detection of histamine released in whole blood samples

Tae Kyu Lim, Hiroko Ohta, Tadashi Matsunaga*

*この研究の対応する著者

研究成果: Article査読

65 被引用数 (Scopus)

抄録

This paper describes an on-chip-type electrochemical flow immunoassay system with a multichanneled matrix column. The multichanneled matrix column was functionally coated with cation-exchange resin and used for separation of proteins. Antihistamine immunoglobulin G (IgG) antibody conjugated with ferrocenemonocarboxylic acid (Fc) was also prepared and used as a novel analytical reagent. Antibody-antigen complexes were separated from free Fc-conjugated IgG antibody (Fc-IgG) on the basis of differences in isoelectric point (pI) using the multichanneled matrix column coated with cation-exchange resin. The assay yields a good relationship between current and histamine concentration in the range of 200-2000 ng/mL. This simple technique enables the assay of histamine released in whole blood within 2 min. Furthermore, a good correlation was found between the response of the electrochemical immunoassay described in this paper and the conventional RIA (radioimmunoassay). This on-chip-type electrochemical flow immunoassay requires only minute quantities of whole blood samples and generates highly reproducible results.

本文言語English
ページ(範囲)3316-3321
ページ数6
ジャーナルAnalytical Chemistry
75
14
DOI
出版ステータスPublished - 2003 7月 15
外部発表はい

ASJC Scopus subject areas

  • 分析化学

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