Microfluidic active sorting of DNA molecules labeled with single quantum dots using flow switching by a hydrogel sol-gel transition

M. Haneoka, Y. Shirasaki, H. Sugino, T. Sekiguchi, D. H. Yoon, R. Iizuka, S. Shoji, T. Funatsu*

*この研究の対応する著者

研究成果: Conference contribution

抄録

A microfluidic device for analyzing and sorting nanometer-sized particles has been developed. To achieve sensitive detection, the sample flow was hydrodynamically sheathed and effectively excited with a focused laser beam. Flow control was performed by a sol-gel transition of a thermoreversible gelation polymer (TGP). Using the developed sorter, Qdot-labeled actin DNA was separated from unwanted DNA, and the purity of Qdot-labeled actin DNA increased following the sorting. This study represents the first example of active separation of biomolecules labeled with single 10 nanometer-sized particles of Qdots.

本文言語English
ホスト出版物のタイトル15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
ページ1773-1775
ページ数3
出版ステータスPublished - 2011
イベント15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
継続期間: 2011 10月 22011 10月 6

出版物シリーズ

名前15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
3

Conference

Conference15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
国/地域United States
CitySeattle, WA
Period11/10/211/10/6

ASJC Scopus subject areas

  • 制御およびシステム工学

フィンガープリント

「Microfluidic active sorting of DNA molecules labeled with single quantum dots using flow switching by a hydrogel sol-gel transition」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル