Microfluidic active sorting of DNA molecules labeled with single quantum dots using flow switching by a hydrogel sol-gel transition

Mai Haneoka, Yoshitaka Shirasaki, Hirokazu Sugino, Tokihiko Aoki, Takahiro Arakawa, Kazuto Ozaki, Dong Hyun Yoon, Noriyuki Ishii, Ryo Iizuka, Shuichi Shoji*, Takashi Funatsu

*この研究の対応する著者

研究成果: Article査読

18 被引用数 (Scopus)

抄録

The fluorescence-activated cell sorter instrument has contributed significantly to life sciences. However, this instrument has limitations including the inability to detect and hence sort nanometer-sized particles such as quantum dots (Qdots) and virus particles. Here, a microfluidic device for analyzing and sorting nanometer-sized particles has been developed. To achieve sensitive detection, the sample flow was hydrodynamically sheathed and effectively excited with a focused laser beam. Flow control was performed by a sol-gel transition of a thermoreversible gelation polymer. This flow control approach enabled us to restrict the lateral diffusion of nanometer-sized particles and to sort the particles in ∼10 μm channels. Single Qdots with diameters of ∼10 nm were detected at a linear flow velocity of about 4 mm/s, and the Qdots were successfully sorted with the sorting system. Using the developed sorter in an application, Qdot-labeled actin DNA was separated from unwanted glyceraldehyde-3-phosphate dehydrogenase DNA, and the purity of Qdot-labeled actin DNA increased following the sorting. This study represents the first example of active separation of biomolecules labeled with single 10 nm-sized particles of Qdots.

本文言語English
ページ(範囲)314-320
ページ数7
ジャーナルSensors and Actuators, B: Chemical
159
1
DOI
出版ステータスPublished - 2011 11月 28

ASJC Scopus subject areas

  • 電子材料、光学材料、および磁性材料
  • 器械工学
  • 凝縮系物理学
  • 表面、皮膜および薄膜
  • 金属および合金
  • 電子工学および電気工学
  • 材料化学

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