Microfluidic alignment system for single cell manipulation and culture

Takahiro Arakawa, Naoya Takeda, Yoshikuni Edagawa, Yoshinori Yamaguchi, S. Shoji

研究成果: Paper査読

抄録

Microfluidic alignment system for single cell manipulation and culture was developed to investigate the differences between individual cells in cell biology. We describe a microfluidic manipulation system that consists of a main channel, a pressure balancing channel (PBC), 3 μm drain channels and semicircle micropockets of 100 μm in diameter, for single cell capture, cell release and cell culture. Fundamental single cell manipulation was obtained by simple flow rate, the ratio between the cells that passed though the injected microchannel and captured cell were about 72%. Multiplication from the single cell in the micropocket was confirmed up to the third generation. This microfluidic system realized noninvasive capture of living single cells, and long-term culture up to 6 days. And this system contributes to the elucidation of cellular nature at the single cell level.

本文言語English
ページ1855-1857
ページ数3
出版ステータスPublished - 2008
イベント12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States
継続期間: 2008 10 122008 10 16

Conference

Conference12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
国/地域United States
CitySan Diego, CA
Period08/10/1208/10/16

ASJC Scopus subject areas

  • 化学工学(その他)
  • バイオエンジニアリング

フィンガープリント

「Microfluidic alignment system for single cell manipulation and culture」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル