MicroRNA-23a has minimal effect on endurance exercise-induced adaptation of mouse skeletal muscle

Shogo Wada, Yoshio Kato, Shuji Sawada, Katsuji Aizawa, Jong Hoon Park, Aaron P. Russell, Takashi Ushida, Takayuki Akimoto*

*この研究の対応する著者

研究成果: Article査読

15 被引用数 (Scopus)

抄録

Skeletal muscles contain several subtypes of myofibers that differ in contractile and metabolic properties. Transcriptional control of fiber-type specification and adaptation has been intensively investigated over the past several decades. Recently, microRNA (miRNA)-mediated posttranscriptional gene regulation has attracted increasing attention. MiR-23a targets key molecules regulating contractile and metabolic properties of skeletal muscle, such as myosin heavy-chains and peroxisome proliferator-activated receptor gamma, coactivator 1 alpha (PGC-1α). In the present study, we analyzed the skeletal muscle phenotype of miR-23a transgenic (miR-23a Tg) mice to explore whether forced expression of miR-23a affects markers of mitochondrial content, muscle fiber composition, and muscle adaptations induced by 4 weeks of voluntary wheel running. When compared with wild-type mice, protein markers of mitochondrial content, including PGC-1α, and cytochrome c oxidase complex IV (COX IV), were significantly decreased in the slow soleus muscle, but not the fast plantaris muscle of miR-23a Tg mice. There was a decrease in type IId/x fibers only in the soleus muscle of the Tg mice. Following 4 weeks of voluntary wheel running, there was no difference in the endurance exercise capacity as well as in several muscle adaptive responses including an increase in muscle mass, capillary density, or the protein content of myosin heavy-chain IIa, PGC-1α, COX IV, and cytochrome c. These results show that miR-23a targets PGC-1α and regulates basal metabolic properties of slow but not fast twitch muscles. Elevated levels of miR-23a did not impact on whole body endurance capacity or exercise-induced muscle adaptations in the fast plantaris muscle.

本文言語English
ページ(範囲)389-398
ページ数10
ジャーナルPflugers Archiv European Journal of Physiology
467
2
DOI
出版ステータスPublished - 2014 2
外部発表はい

ASJC Scopus subject areas

  • 生理学
  • 臨床生化学
  • 生理学(医学)

フィンガープリント

「MicroRNA-23a has minimal effect on endurance exercise-induced adaptation of mouse skeletal muscle」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル