Microscopic analysis of polymerization dynamics with individual actin filaments

Ikuko Fujiwara, Shin Takahashi, Hisashi Tadakuma, Takashi Funatsu, Shin'ichi Ishiwata*

*この研究の対応する著者

    研究成果: Article査読

    156 被引用数 (Scopus)

    抄録

    The polymerization-depolymerization dynamics of actin is a key process in a variety of cellular functions. Many spectroscopic studies have been performed in solution, but studies on single actin filaments have just begun. Here, we show that the time course of polymerization of individual filaments consists of a polymerization phase and a subsequent steady-state phase. During the steady-state phase, a treadmilling process of elongation at the barbed end and shortening at the pointed end occurs, in which both components of the process proceed at approximately the same rate. The time correlation of length fluctuation of the filaments in the steady-state phase showed that the polymerization-depolymerization dynamics follow a diffusion (stochastic) process, which cannot be explained by simple association and dissociation of monomers at both ends of the filaments.

    本文言語English
    ページ(範囲)666-673
    ページ数8
    ジャーナルNature Cell Biology
    4
    9
    DOI
    出版ステータスPublished - 2002 9月

    ASJC Scopus subject areas

    • 細胞生物学

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