Microscopic heat pulses induce contraction of cardiomyocytes without calcium transients

Kotaro Oyama, Akari Mizuno, Seine A. Shintani, Hideki Itoh, Takahiro Serizawa, Norio Fukuda, Madoka Suzuki, Shin'ichi Ishiwata

研究成果: Article査読

25 被引用数 (Scopus)

抄録

It was recently demonstrated that laser irradiation can control the beating of cardiomyocytes and hearts, however, the precise mechanism remains to be clarified. Among the effects induced by laser irradiation on biological tissues, temperature change is one possible effect which can alter physiological functions. Therefore, we investigated the mechanism by which heat pulses, produced by infra-red laser light under an optical microscope, induce contractions of cardiomyocytes. Here we show that microscopic heat pulses induce contraction of rat adult cardiomyocytes. The temperature increase, ΔT, required for inducing contraction of cardiomyocytes was dependent upon the ambient temperature; that is, ΔT at physiological temperature was lower than that at room temperature. Ca 2+ transients, which are usually coupled to contraction, were not detected. We confirmed that the contractions of skinned cardiomyocytes were induced by the heat pulses even in free Ca 2+ solution. This heat pulse-induced Ca 2+-decoupled contraction technique has the potential to stimulate heart and skeletal muscles in a manner different from the conventional electrical stimulations.

本文言語English
ページ(範囲)607-612
ページ数6
ジャーナルBiochemical and Biophysical Research Communications
417
1
DOI
出版ステータスPublished - 2012 1 6

ASJC Scopus subject areas

  • 生化学
  • 生物理学
  • 細胞生物学
  • 分子生物学

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