Microtubule-bundling activity of the centrosomal protein, Cep169, and its binding to microtubules

Yusuke Mori, Yuki Taniyama, Sayori Tanaka, Hiroki Fukuchi, Yasuhiko Terada*

*この研究の対応する著者

研究成果: Article査読

3 被引用数 (Scopus)

抄録

CDK5RAP2 is a centrosomal protein that regulates the recruitment of a γ-tubulin ring complex (γ-TuRC) onto centrosomes and microtubules (MTs) dynamics as a member of MT plus-end-tracking proteins (+TIPs). In our previous report, we found mammalian Cep169 as a CDK5RAP2 binding partner, and Cep169 accumulates at the distal ends of MTs and centrosomes, and coincides with CDK5RAP2. Depletion of Cep169 induces MT depolymerization, indicating that Cep169 targets MT tips and regulates stability and dynamics of MTs. However, how Cep169 contributes to the stabilization of MT remains unclear. Here we show that Cep169 is able to stabilize MTs and induces formation of long MT bundles with intense acetylation of MTs with CDK5RAP2, when expressed at higher levels in U2OS cells. In addition, we demonstrated that Cep169 forms homodimers through its N-terminal domain and directly interacts with MTs through its C-terminal domain. Interestingly, Cep169 mutants, which lack each domains, completely abolished the activity, respectively. Therefore, Cep169 bundles MTs and induces solid structure of MTs by crosslinking each adjacent MTs as a homodimer.

本文言語English
ページ(範囲)754-759
ページ数6
ジャーナルBiochemical and Biophysical Research Communications
467
4
DOI
出版ステータスPublished - 2015 11 27

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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