Microtubules and Alp7-Alp14 (TACC-TOG) reposition chromosomes before meiotic segregation

Yasutaka Kakui, Masamitsu Sato, Naoyuki Okada, Takashi Toda, Masayuki Yamamoto

研究成果: Article

19 引用 (Scopus)

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Tethering kinetochores at spindle poles facilitates their efficient capture and segregation by microtubules at mitotic onset in yeast. During meiotic prophase of fission yeast, however, kinetochores are detached from the poles, which facilitates meiotic recombination but may cause a risk of chromosome mis-segregation during meiosis. How cells circumvent this dilemma remains unclear. Here we show that an extensive microtubule array assembles from the poles at meiosis I onset and retrieves scattered kinetochores towards the poles to prevent chromosome drift. Moreover, the microtubule-associated protein complex Alp7-Alp14 (the fission yeast orthologues of mammalian TACC-TOG) is phosphorylated by Polo kinase, which promotes its meiosis-specific association to the outer kinetochore complex Nuf2-Ndc80 of scattered kinetochores, thereby assisting in capturing remote kinetochores. Although TOG was recently characterized as a microtubule polymerase, Dis1 (the other TOG orthologue in fission yeast), together with the Dam1 complex, plays a role in microtubule shortening to pull kinetochores polewards. Thus, microtubules and their binding proteins uniquely reconstitute chromosome configuration during meiosis.

元の言語English
ページ(範囲)786-796
ページ数11
ジャーナルNature Cell Biology
15
発行部数7
DOI
出版物ステータスPublished - 2013 7 1

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ASJC Scopus subject areas

  • Cell Biology

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