Miniaturized amperometric flow immunoassay system using a glass fiber membrane modified with anion

Tae Kyu Lim, Satoko Imai, Tadashi Matsunaga

研究成果: Article

17 被引用数 (Scopus)

抄録

This paper describes a miniaturized amperometric flow immunoassay system using a glass fiber membrane modified with anion. The glass fiber membrane was functionally modified with γ-glycidoxypropyltrimethoxysilane and sodium thiosulfate and was used for separation of protein. Anti-human chorionic gonadotrophin (HCG) immunoglobulin G (IgG) antibody conjugated with ferrocenemonocarboxylic acid (Fc), namely, Fc-conjugated IgG (Fc-IgG), was used as a novel analytical reagent. HCG and Fc-IgG complexes were separated from free Fc-IgG based on differences in isoelectric point (pl) using the glass fiber membrane modified with a thiosulfonyl acid functional group. The assay yields a linear relationship between current and HCG concentration in the range of 0-2000 mlU/mL. This simple technique enables the assay of HCG within 2 min. The modified glass fiber membrane was regenerated by occasional elution with malonate buffer (pH 6.0) containing 0.5 M NaCl, to remove free Fc-IgG. Free Fc-IgG recovered in this manner could be reused up to eight times without significant decreases in sensitivity. This miniaturized amperometric flow immunoassay requires only minute quantities of serum and generates highly reproducible results.

本文言語English
ページ(範囲)758-763
ページ数6
ジャーナルBiotechnology and Bioengineering
77
7
DOI
出版ステータスPublished - 2002 3 30
外部発表はい

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology

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